Pharmacokinetics and tissue distribution of four alkaloids in Ermiao Pills and Sanmiao Pills in normal and arthritic model rats.
10.19540/j.cnki.cjcmm.20221224.501
- Author:
Bing-Jie LI
1
;
Wen-Jing GE
2
;
Peng-Tao SHAN
1
;
Hui-Sen WANG
2
;
Ming LIU
2
;
Geng-Sheng LI
2
;
Rui-Feng LIANG
1
Author Information
1. School of Pharmacy, Henan University of Chinese Medicine Zhengzhou 450046, China Institute of Chinese Materia Medica, Henan Academy of Chinese Medicine Zhengzhou 450004, China.
2. Institute of Chinese Materia Medica, Henan Academy of Chinese Medicine Zhengzhou 450004, China.
- Publication Type:Journal Article
- Keywords:
Achyranthis Bidentatae Radix;
Ermiao Pills;
Sanmiao Pills;
guiding meridian;
pharmacokinetics;
tissue distribution
- MeSH:
Rats;
Animals;
Berberine/pharmacokinetics*;
Tissue Distribution;
Chromatography, Liquid;
Tandem Mass Spectrometry/methods*;
Drugs, Chinese Herbal/pharmacokinetics*;
Alkaloids/pharmacokinetics*;
Chromatography, High Pressure Liquid/methods*;
Arthritis
- From:
China Journal of Chinese Materia Medica
2023;48(7):1943-1950
- CountryChina
- Language:Chinese
-
Abstract:
This work aimed to investigate the differences of pharmacokinetics and tissue distribution of four alkaloids in Ermiao Pills and Sanmiao Pills in normal and arthritic model rats. The rat model of arthritis was established by injecting Freund's complete adjuvant, and ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) in the positive ion multiple reaction monitoring(MRM) mode was used for the determination of four alkaloids in plasma and tissues of normal and arthritic rats after administration of Ermiao Pills and Sanmiao Pills, respectively. The differences in pharmacokinetics and tissue distribution of the four active components were compared, and the effect of Achyranthis Bidentatae Radix on the major components of Sanmiao Pills was explored. This study established an UPLC-MS/MS for simultaneous determination of four alkaloids, and the specificity, linearity, accuracy, precision, and stability of this method all met the requirements. Pharmacokinetics study found that as compared with normal rats, the AUC and C_(max) of phellodendrine, magnoflorine, berberine and palmatine in model rats were significantly decreased after administration of Ermiao Pills, the clearance rate CL/F was significantly increased, and the distribution and tissue/plasma concentration ratio of the four alkaloids in the liver, kidney, and joint were significantly reduced. Achyranthis Bidentatae Radix increased the AUC of phellodendrine, berberine, and palmatine, reduced the clearance rate, and significantly increased the distribution of the four alkaloids in the liver, kidney, and joints in arthritic rats. However, it had no significant effect on the pharmacokinetics and tissue distribution of the four alkaloids in normal rats. These results suggest that Achyranthis Bidentatae Radix may play a guiding role in meridian through increasing the tissue distribution of effective components in Sanmiao Pills under arthritis states.
