Exploring the key anti-fatigue components of American ginseng based on metabolomics and zebrafish models
10.16438/j.0513-4870.2022-1277
- VernacularTitle:基于代谢组学和斑马鱼模型探究西洋参抗疲劳的关键活性成分
- Author:
Hui-ru LIU
1
;
Hai-yang WANG
2
;
Zhe WANG
2
;
Li-guo CHEN
2
;
Gui-dong CHENG
2
;
Zhi-hui MA
1
;
Song-song WANG
3
;
Zheng-guo LI
4
;
Li-wen HAN
2
Author Information
1. College of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China; School of Pharmacy and Pharmaceutical Sciences & Institute of Materia Medica, Shandong First Medical University & Shandong Academy of Medical Sciences, NHC Key Laboratory of Biotechnology Drugs (Shandong Academy of Medical Sciences), Jinan 250000, China
2. School of Pharmacy and Pharmaceutical Sciences & Institute of Materia Medica, Shandong First Medical University & Shandong Academy of Medical Sciences, NHC Key Laboratory of Biotechnology Drugs (Shandong Academy of Medical Sciences), Jinan 250000, China
3. College of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China
4. College of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China; Jining Food and Drug Inspection and Testing Research Institute, Jining 272027, China
- Publication Type:Research Article
- Keywords:
metabolomics;
American ginseng;
anti-fatigue;
zebrafish;
pseudoginsenoside F11
- From:
Acta Pharmaceutica Sinica
2023;58(4):1024-1032
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this paper is to explore the key anti-fatigue active components in the saponin-like composition of American ginseng. The anti-fatigue activity of western ginseng samples was evaluated using a zebrafish model; metabolomics techniques were used to identify the main saponins in western ginseng from different origins; the active substances and relevant targets of the anti-fatigue effect of western ginseng were initially screened by constructing a PPI protein interaction network between western ginseng saponins and disease targets, and the key active ingredients were screened using a molecular docking method; finally, the anti-fatigue activity of the key active ingredients was evaluated using a zebrafish, animal experiment was approved by the Ethics Committee of Shandong Academy of Medical Sciences (SYXK20220005). The anti-fatigue activity of the key active ingredients was evaluated using a zebrafish model. The results of the zebrafish activity evaluation showed that there were significant differences in the activities of the western ginseng samples from the two origins, and a total of 10 different saponins were identified as possibly related to the anti-fatigue activity after further metabolomic testing and pattern discrimination. The core anti-fatigue targets were screened with the help of component-disease target PPI, combined with pharmacophore-like parameters and molecular docking techniques, and pseudoginsenoside F11 was found to have good binding activity to five of the targets. Finally, the zebrafish model revealed that pseudoginsenoside F11 exhibited significant anti-fatigue activity. This study used metabolomics and zebrafish model to screen the key active substances of pseudoginsenoside F11 for its anti-fatigue activity, which will provide a reference for further research on the anti-fatigue of pseudoginsenosides.
