Effects of propofol on glutamatergic neuronal activity in paraventricular thalamus
10.16438/j.0513-4870.2022-0802
- VernacularTitle:丙泊酚对丘脑室旁核谷氨酸能神经元活性的影响
- Author:
Yu-long WANG
1
;
Qiong YI
2
;
Wei XU
3
;
E WANG
2
;
Zhi-li HUANG
3
;
Yong-quan CHEN
1
;
Lu WANG
3
Author Information
1. Yijishan Hospital, Wannan Medical College, Wuhu 241000, China
2. Xiangya Hospital, Central South University, Changsha 410008, China
3. State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science, School of Basic Medical Sciences, Shanghai Medical College of Fudan University, Shanghai 200032, China
- Publication Type:Research Article
- Keywords:
propofol;
paraventricular thalamus;
hole-cell patch-clamp;
gamma-aminobutyric acid type A receptor;
miniature inhibitory postsynaptic current
- From:
Acta Pharmaceutica Sinica
2023;58(4):919-927
- CountryChina
- Language:Chinese
-
Abstract:
This study explored the effects of propofol on the activity of glutamatergic neurons in the paraventricular thalamus (PVT) and the underlying mechanisms at the molecular level using whole-cell patch-clamp techniques. Acute brain slices containing the PVT were obtained from 8 weeks old C57BL/6J mice. The electrophysiological characteristics of PVT neurons were recorded in current-clamp mode, then single-cell sequencing was used to identify neuronal types. The firing frequencies before, during, and after propofol or intralipid application were recorded as FB, FD and FW; and the membrane potentials were recorded as MPB and MPD. Picrotoxin (PTX) was used to block inhibitory gamma-aminobutyric acid type A (GABAA) receptors during the application of propofol at 10 μmol·L-1. Then, GABAA receptor-mediated spontaneous and miniature inhibitory postsynaptic currents (sIPSCs and mIPSCs) were recorded, and the effects of 10 μmol·L-1 propofol were investigated. The animal experiments were approved by the Medical Animal Administrative Committee of Shanghai Medical College Fudan University. The results showed that there were no significant differences in FB, FD and FW during intralipid and 2 μmol·L-1 propofol application. With propofol at 5, 10 and 20 μmol·L-1, FD decreased significantly when compared with FB, and FW increased significantly as compared with FD (P < 0.01). The inhibition degree of the three concentration groups was significantly different (P < 0.01). In addition, with propofol at 20 μmol·L-1, MPD hyperpolarized significantly (P < 0.01). In the presence of PTX, 10 μmol·L-1 propofol could not suppress the firing frequency of PVT glutamatergic neurons. Propofol at 10 μmol·L-1 prolonged the decay time of sIPSCs (P < 0.01) and mIPSCs (P < 0.05), and increased the amplitude (P < 0.01) of mIPSCs of PVT glutamatergic neurons. Together, these results indicate that propofol can inhibit the activity of PVT glutamatergic neurons in a concentration-dependent and reversible manner, and the effect is likely to be mediated by postsynaptic GABAA receptors.
