Analysis of Fusarium oxysporum infection on changes of Astragalus metabolism by metabolomic approach
10.16438/j.0513-4870.2022-1377
- VernacularTitle:基于代谢组学分析尖孢镰刀菌侵染对黄芪代谢的影响
- Author:
Fu-xin WANG
1
;
Hai-qing SONG
1
;
Li ZHAO
1
;
Xue-mei QIN
1
;
Fen GAO
2
;
Zhen-yu LI
1
Author Information
1. Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan 030006, China
2. Institute of Applied Chemistry, Shanxi University, Taiyuan 030006, China
- Publication Type:Research Article
- Keywords:
italic>Astragalus membranaceus var. mongholicus;
root rot;
metabolite;
italic>Fusarium oxysporum;
flavonoid
- From:
Acta Pharmaceutica Sinica
2023;58(7):1971-1980
- CountryChina
- Language:Chinese
-
Abstract:
italic>Astragalus is a commonly used Chinese medicinal material in traditional Chinese medicine (TCM), and with the increase of planting area in recent years, the damage of Astragalus root rot has worsened year by year, which seriously affecting its quality and yield. Fusarium oxysporum is one of the main pathogens causing root rot in astragalus. In this study, UPLC-Q-TOF-MS based metabolomic approach combined with multivariate statistical analysis were used to analyze the metabolite changes of Astragalus in response to F. oxysporum infection. The results showed that 62 metabolites in the Astragalus had significant changes after inoculation of F. oxysporum. Polar metabolites included 40 flavonoids, 8 saponins, 2 nucleosides, 1 vitamin, 1 organic acid, 1 amino acid; while lipid metabolites included 3 fatty acids, 1 diradylglycerols, 2 lysophosphatidylcholine, 1 lysophosphatidylglycerol, 1 phosphatidylinositol, 1 sterol lipid. Among these differential metabolites, the relative content of flavonoids, vitamin B2, tryptophan and salicylic acid were increased, while the relative content of saponins were decreased. Correlation analysis showed that the flavonoids were positively correlated with each other, and positively correlated with most lipids, but negatively correlated with most saponins. In addition, studies have shown that F. oxysporum infection is not an influencing factor for the generation of malonyl substitution of flavonoid. This study elucidates the effect of F. oxysporum infection on Astragalus from the perspective of plant metabolism, which provides a basis for exploring the interaction mechanism between the Astragalus and F. oxysporum and further promoting molecular breeding.
