Mechanism of Banxia Baizhu Tianma decoction to prevent the development of prehypertension and excessive phlegm-dampness syndrome in rats based on metabonomics technology
10.16438/j.0513-4870.2022-1216
- VernacularTitle:基于代谢组学技术探究半夏白术天麻汤对正常高值血压痰湿壅盛证大鼠的干预机制
- Author:
Jian-qiao YE
1
;
Xiao-qing GUO
1
;
Shu-min LI
2
;
Hai-qiang JIANG
3
;
Dong-mei QI
4
;
Yu-chen QI
5
Author Information
1. Institute of Innovation in Traditional Chinese Medicine, Shandong University of Traditional Chinese Medicine, Jinan 250355, China
2. Experimental Center, Shandong University of Traditional Chinese Medicine, Jinan 250355, China
3. Institute of Innovation in Traditional Chinese Medicine, Shandong University of Traditional Chinese Medicine, Jinan 250355, China; Shandong Provincial Key Laboratory of Traditional Chinese Medicine for Basic Research, Jinan 250355, China; Key Laboratory of Traditional Chinese Medicine Classical Theory, Ministry of Education, Shandong University of Traditional Chinese Medicine, Jinan 250355, China
4. Experimental Center, Shandong University of Traditional Chinese Medicine, Jinan 250355, China; Shandong Provincial Key Laboratory of Traditional Chinese Medicine for Basic Research, Jinan 250355, China; Key Laboratory of Traditional Chinese Medicine Classical Theory, Ministry of Education, Shandong University of Traditional Chinese Medicine, Jinan 250355, China
5. Faculty of Health, Shandong University of Traditional Chinese Medicine, Jinan 250355, China
- Publication Type:Research Article
- Keywords:
Banxia Baizhu Tianma decoction;
prehypertension;
phlegm-dampness syndrome;
metabonomics
- From:
Acta Pharmaceutica Sinica
2023;58(7):1822-1832
- CountryChina
- Language:Chinese
-
Abstract:
The alterations of serum biological endogenous chemicals in rats with phlegm dampness accumulation syndrome of prehypertension (PHT) were interfered by Banxia Baizhu Tianma decoction (BBT), and the metabolic regulatory pathway of BBT was clarified using serum metabonomics analysis. To replicate the rat model of prehypertension phlegm dampness syndrome, blood pressure, behavioral markers, and serum biochemical markers of rats were collected. BBT's effectiveness in controlling blood pressure and blood lipids was assessed, and changes in endogenous small molecules in rat serum were determined using UPLC-Q-Orbitrap MS metabolic analysis. The results showed that BBT could regulate 9 metabolites, including arachidonic acid, cholic acid, glycodeoxycholic acid, N-adenosyltyrosine, arginine, lysophosphatidylethanolamine (20:0/0:00), lysophospholipid (P-18:0), lysophospholipid (18:0), lysophospholipid (22:5(7Z,10Z,13Z,16Z,19Z)). MetaboAnalyst was used to analyze the metabolic pathway. There were 7 metabolic pathways closely related to the change of blood pressure in rats, among which arachidonic acid metabolic pathway was the most critical. The metabolism difference foreign body in the model rats tends to return to the normal level, which provides a research basis for the mechanism of BBT from the perspective of metabonomics. This study was approved by the Experimental Animal Welfare Ethics Review Committee of Shandong University of Traditional Chinese Medicine (approval number: SDUTCM20211103001).
