Combined Therapy with Mixed Heat Shock Protein/Peptide Vaccine and Interleukin-12 Results in Enhanced Antitumor Effects
- VernacularTitle:混合性热休克蛋白/肽疫苗与白细胞介素-12联合诱导特异性细胞毒T淋巴细胞产生的实验研究
- Author:
Ge SONG
;
Mei YUAN
;
Xuemei CUI
;
Xiang SUI
;
Bin ZHAO
;
Shibi LU
- Publication Type:Journal Article
- Keywords:
sarcoma-180, neoplasm, immunotherapy, mixed heat shock proteins/peptide complexes, cytotoxic T lymphocyte (CTL), vaccine
- From:
Chinese Journal of Rehabilitation Theory and Practice
2007;13(3):252-254
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the antitumor immunity induced by tumor derived mixed heat shock protein/peptide(mHSPs),interleukin-12(IL-12)and Cyclophosphamide(Cy).MethodsPurified mixed HSP was prepared from tumor by S180 protein extraction and purification,SDS-PAGE,Western blot and animal experiment were applied for mixed HSPs analysis.ResultsThe proliferation of cytotoxic T lymphocyte(CTL)cocultured in the mHSPs+Cy+IL-12 group was significantly remarkable and the content of CD8+ CTLs was significantly more in comparison with the other groups(P<0.01).To the tumor bearing mice,mHSPs+Cy+IL-12 group showed partial therapeutic efficacy,the averaged survival period was over 60 d,and 90% of the mice in this group got long period tumor free survival(>90d),obvious difference(P<0.05)from the other groups.ConclusionTumor derived mixed HSPs can induce powerful antitumor immune efficacy and show favorable therapeutic efficacy.
