Construction and in vitro evaluation of an LNP system for mRNA delivery

VernacularTitle:mRNA脂质纳米粒载药系统的构建及体外评价
Author: Xinlu CHEN ¹ ; Yuan GAO ² ; Juanjuan LI ¹ ; Huanhuan GUO ¹ ; Zhuo WANG ¹ ; Shen GAO ¹
Author Information

1. The First Affiliated Hospital, Naval Medical University, Shanghai 200433, China.
2. School of Pharmacy, Fudan University, Shanghai 200032, China.
Keywords: LNP; DLin-MC3-DMA; mRNA; drug delivery
From: Journal of Pharmaceutical Practice 2023;41(5):291-295
CountryChina
Language:Chinese
Abstract: Objective To construct lipid nanoparticles DLin-LNP for mRNA delivery. Methods DLin-LNP was prepared by thin film hydration method, and DLin-LNP/mRNA was further constructed by using EGFP-mRNA as model drug. The particle size, zeta potential, and appearance morphology were measured. Furthermore, the intracellular distribution and transfection of DLin-LNP/mRNA in RM-1 cells was investigated by laser scanning confocal microscope. Results DLin-LNP was successfully prepared. The average particle size was about (151.1±2.1) nm, the no-load potential was (23.7±0.5) mV. The cytotoxicity of DLin-LNP was far lower than that of the commercially available liposomal Lipo8000. The results of transfection experiment indicated that DLin-LNP has high transfection efficiency for mRNA delivery with low cytotoxicity and good stability. Conclusion DLin-LNP could become a potential mRNA vector for gene therapy.