Sevoflurane pretreatment inhibits the myocardial apoptosis caused by hypoxia reoxygenation through AMPK pathway: An experimental study
10.1016/j.apjtm.2017.01.006
- Author:
Zhao-Ying LIU
1
;
Shun-Peng HU
1
;
Qing-Rong JI
1
;
Hai-Bo YANG
1
;
Fang-Fang WU
1
;
Dong-Hao ZHOU
2
Author Information
1. Department of Cardiology, Linyi People's Hospital in Shandong Province
2. Department of Endocrinology, Linyi People's Hospital in Shandong Province
- Publication Type:Journal Article
- Keywords:
Adenosine;
Apoptosis;
Monophosphate-activated;
Myocardial hypoxia reoxygenation;
Protein kinase;
Sevoflurane
- From:
Asian Pacific Journal of Tropical Medicine
2017;10(2):148-151
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study whether sevoflurane pretreatment inhibits the myocardial apoptosis caused by hypoxia reoxygenation through AMPK pathway. Methods H9c2 myocardial cell lines were cultured and divided into control group (C group), hypoxia reoxygenation group (H/R group), sevoflurane pretreatment + hypoxia reoxygenation group (SP group) and sevoflurane combined with Compound C pretreatment + hypoxia reoxygenation group (ComC group), and the cell proliferation activity and apoptosis rate, myocardial enzyme levels in culture medium as well as the expression of apoptosis genes and p-AMPK in cells were determined. Results p-AMPK expression in cells of H/R group was significantly lower than that of C group, SP group was significantly higher than that of H/R group; cell proliferation activity value and Bcl-2 expression in cells of H/R group were significantly lower than those of C group, SP group were significantly higher than those of H/R group, ComC group were significantly lower than those of SP group; apoptosis rate, LDH, CK and AST levels as well as the Bax and Caspase-3 expression in cells of H/R group were significantly higher than those of C group, SP group were significantly lower than those of H/R group, ComC group were significantly higher than those of SP group. Conclusions Sevoflurane pretreatment can activate AMPK signaling pathway to inhibit the myocardial apoptosis caused by hypoxia reoxygenation.
