Rosmarinic acid attenuates hepatic fibrogenesis via suppression of hepatic stellate cell activation/proliferation and induction of apoptosis
10.1016/j.apjtm.2017.05.012
- Author:
Naglaa M. EL-LAKKANY
1
;
Walaa H. EL-MAADAWY
1
;
Sayed H. SEIF EL-DIN
1
;
Olfat A. HAMMAM
2
;
Salwa H. MOHAMED
3
;
Shahira M. EZZAT
4
;
Marwa M. SAFAR
5
;
Samira SALEH
5
Author Information
1. Department of Pharmacology, Theodor Bilharz Research Institute, Warak El-Hadar
2. Department of Pathology, Theodor Bilharz Research Institute, Warak El-Hadar
3. Department of Immunology, Theodor Bilharz Research Institute, Warak El-Hadar
4. Department of Pharmacognosy, Faculty of Pharmacy, Cairo University
5. Department of Pharmacology and Toxicology, Faculty of Pharmacy, Cairo University
- Publication Type:Journal Article
- Keywords:
Apoptosis;
Hepatic fibrosis;
Hepatic stellate cells;
Profibrogenic markers;
Proliferation;
Rosmarinic acid
- From:
Asian Pacific Journal of Tropical Medicine
2017;10(5):444-453
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the antifibrotic role of rosmarinic acid (RA), a natural polyphenolic compound, on HSCs activation/proliferation and apoptosis in vitro and in vivo. Methods The impact of RA on stellate cell line (HSC-T6) proliferation, activation and apoptosis was assessed along with its safety on primary hepatocytes. In vivo, rats were divided into: (i) normal; (ii) thioacetamide (TAA)-intoxicated rats for 12 weeks; (iii) TAA + silymarin or (iv) TAA + RA. At the end of experiment, liver functions, oxidative stress, inflammatory and profibrogenic markers, tissue inhibitor metalloproteinases type-1 (TIMP-1) and hydroxyproline (HP) levels were evaluated. Additionally, liver histopathology and immunohistochemical examinations of alpha-smooth muscle actin (α-SMA), caspase-3 and proliferation cellular nuclear antigen (PCNA) were determined. Results RA exhibited anti-proliferative effects on cultured HSCs in a time and concentration dependent manner showing an IC
