Inhibitory effects of gemcitabine hydrochloride combined with ferroptosis inducers on the proliferation of PANC-1 cells
10.12206/j.issn.2097-2024.202212051
- VernacularTitle:盐酸吉西他滨联合铁死亡诱导剂对PANC-1细胞的增殖抑制作用
- Author:
Jirong WANG
1
;
Daoqiu YANG
2
;
Zhiguo SUN
1
;
Han HAN
3
;
Li DENG
1
Author Information
1. Department of Pharmaceutics, School of Pharmacy, Naval Medical University, Shanghai 200433, China.
2. Department of Dermatology, No. 970 Hospital of Joint Logistics Support Force of the PLA, Yantai 264002, China.
3. Jingzhong Medical District, General Hospital of the PLA, Beijing 100820, China.
- Keywords:
ferroptosis inducer;
gemcitabine hydrochloride;
pancreatic cancer;
synergistic inhibition
- From:
Journal of Pharmaceutical Practice
2023;41(4):234-239
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of three ferroptosis inducers Erastin (Era), sulfasalazine (SASP) and artesunate (Art) alone or combined with gemcitabine hydrochloride (hcGEM) on the proliferation inhibition of Human pancreatic cell line PANC -1. Methods The CCK-8 method was used to detect the inhibitory effects of different concentrations of Era, SASP and Art alone or combined with hcGEM on the proliferation of PANC-1, and the combination index (CI) was used to judge whether three ferroptosis inducers combined with hcGEM had synergistic inhibitory effect on PANC-1. Results The three ferroptosis inducers and hcGEM alone or in combination could significantly inhibit the activity of PANC-1. The inhibitory effects were enhanced with the concentration increasing. The CI values of hcGEM-Era 4∶1 or 1∶4 combination group and hcGEM-SASP 1∶400 combination group were less than 1.The CI values of hcGEM-Art 1∶4 or 1∶16 combination group were less than 1 only within a certain concentration range. Conclusion The inhibitory effects of the three ferroptosis inducers and hcGEM alone or in combination were dose-dependent. The combination of hcGEM and three ferroptosis inducers could synergistically inhibit the proliferation of PANC-1.
