Involvement of Interleukin-1 β/Insulin-Like Growth Factor 1 in Ameliorating Effects of Electroacupuncture on Myocardial Fibrosis Induced by Essential Hypertension.
10.1007/s11655-022-2897-0
- Author:
Juan-Juan XIN
1
;
Jun-Hong GAO
1
;
Qun LIU
1
;
Yu-Xue ZHAO
1
;
Chen ZHOU
1
;
Xiao-Chun YU
2
Author Information
1. Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing, 100700, China.
2. Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing, 100700, China. yuxc@sina.com.
- Publication Type:Journal Article
- Keywords:
electroacupuncture;
insulinlike growth factor 1;
interleukin-1 β;
myocardial fibrosis;
spontaneously hypertensive rats
- MeSH:
Rats;
Animals;
Male;
Rats, Inbred WKY;
Electroacupuncture;
Hypertension/therapy*;
Insulin-Like Growth Factor I;
Interleukin-1beta;
Rats, Inbred SHR;
Essential Hypertension;
Myocardium/pathology*;
Collagen Type I;
Fibrosis
- From:
Chinese journal of integrative medicine
2023;29(2):162-169
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:To investigate the effect of electroacupuncture (EA) at Neiguan (PC 6) on myocardial fibrosis in spontaneously hypertensive rats (SHRs), and to explore the contribution of interleukin-1 β (IL-1 β), insulin-like growth factor 1 (IGF-1), and transforming growth factor β 1 (TGF- β 1) to the effects.
METHODS:Nine 12-weeks-old Wistar Kyoto (WKY) male rats were employed as the normal group. Twenty-seven SHRs were equally randomized into SHR, SHR+EA, and SHR + sham groups. EA was applied at bilateral PC 6 once a day 30 min per day in 8 consecutive weeks. After 8-weeks EA treatment at PC 6, histopathologic changes of collagen type I (Col I), collagen type 1 (Col 1) and the levels of IGF-1, 1L-1 β, TGF- β 1, matrix metalloproteinase (MMP)-2 and MMP-9 were examined in myocardial tissure respectively.
RESULTS:After 8-weeks EA treatment at PC 6, the enhanced myocardial fibrosis in SHRs were characterized by the increased mean fluorescence intensity of Col I and Col 1 in myocardium tissue (P<0.01). All these abnormal alterations above in SHR + EA group was significantly lower compared with the SHR group (P<0.01). Meanwhile, the increased levels of IL-1 β, IGF-1, TGF-β 1 in serum or myocardial tissue of SHRs, diminished MMP 9 mRNA expression in SHRs were also markedly inhibited after 8 weeks of EA treatment (P<0.05 or P<0.01). Furthermore, the contents of IL-1 β, IGF-1, TGF-β 1 in myocardial tissue were positively correlated with the systolic blood pressure and hydroxyproline respectively (P<0.01).
CONCLUSION:EA at bilateral PC 6 could ameliorate cardiac fibrosis in SHRs, which might be mediated by regulation of 1L-1 β/IGF-1-TGF- β 1-MMP9 pathway.
