Blood metabolites in preterm infants with retinopathy of prematurity based on tandem mass spectrometry: a preliminary study.
10.7499/j.issn.1008-8830.2209142
- Author:
Qiu-Ping YANG
1
;
Si-Tao LI
1
;
Hu HAO
1
;
Xia GU
1
;
Cong-Cong SHI
;
Xin XIAO
1
;
Yao CAI
1
Author Information
1. Department of Pediatrics, Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou 510655, China.
- Publication Type:Journal Article
- Keywords:
Biomarker;
Liquid chromatography;
Metabolomics;
Preterm infant;
Retinopathy of prematurity;
Tandem mass spectrometry
- MeSH:
Infant, Newborn;
Infant;
Humans;
Tandem Mass Spectrometry;
Infant, Premature;
Chromatography, Liquid;
Retinopathy of Prematurity/diagnosis*;
Glutamic Acid;
Ornithine
- From:
Chinese Journal of Contemporary Pediatrics
2023;25(2):140-146
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVES:To study new biomarkers for the early diagnosis of retinopathy of prematurity (ROP) by analyzing the differences in blood metabolites based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) and metabolomics.
METHODS:Dried blood spots were collected from 21 infants with ROP (ROP group) and 21 infants without ROP (non-ROP group) who were hospitalized in the Sixth Affiliated Hospital of Sun Yat-sen University from January 2013 to December 2016. LC-MS/MS was used to measure the metabolites, and orthogonal partial least squares-discriminant analysis was used to search for differentially expressed metabolites and biomarkers.
RESULTS:There was a significant difference in blood metabolic profiles between the ROP and non-ROP groups. The pattern recognition analysis, Score-plot, and weight analysis obtained 10 amino acids with a relatively large difference. Further statistical analysis showed that the ROP group had significant increases in blood levels of glutamic acid, leucine, aspartic acid, ornithine, and glycine compared with the non-ROP group (P<0.05). The receiver operating characteristic curve analysis showed that glutamic acid and ornithine had the highest value in diagnosing ROP.
CONCLUSIONS:Blood metabolites in preterm infants with ROP are different from those without ROP. Glutamic acid and ornithine are the metabolic markers for diagnosing ROP. LC-MS/MS combined with metabolomics analysis has a potential application value in the early identification and diagnosis of ROP.
