Mechanism of Qiwei Guibao Granules in treatment of premature ovarian failure based on proteomics.
10.19540/j.cnki.cjcmm.20221111.701
- Author:
Xue-Ling LIU
1
;
Wei-Wei MAO
1
;
Zi-Xin ZHOU
1
;
Zhong-Kun XU
2
;
Wen-Hua TAO
3
;
Xiao-Yi ZHU
3
;
Hua QIAN
3
;
Qi GUO
1
Author Information
1. School of Medicine, Jiangsu University Zhenjiang 212013, China.
2. Jiangsu Kanion Pharmaceutical Co., Ltd. Lianyungang 222047, China.
3. Department of Traditional Chinese Medicine, Affiliated Hospital of Jiangsu University Zhenjiang 212000, China.
- Publication Type:Journal Article
- Keywords:
Qiwei Guibao Granules;
STAR;
XAF1;
premature ovarian failure;
proteomics
- MeSH:
Female;
Humans;
Mice;
Animals;
Primary Ovarian Insufficiency/chemically induced*;
Proteomics;
Signal Transduction;
Glycosides/adverse effects*
- From:
China Journal of Chinese Materia Medica
2023;48(5):1310-1318
- CountryChina
- Language:Chinese
-
Abstract:
In this study, the underlying mechanism of Qiwei Guibao Granules(QWGB) in the treatment of premature ovarian fai-lure(POF) was explored by the proteomics technique. Firstly, the POF model was induced in mice by intragastric administration of Tripterygium wilfordii glycosides solution at 50 mg·kg~(-1) for 14 days. Ten days prior to the end of the modeling, the estrous cycle of mice was observed every day to evaluate the success of modeling. From the 1st day after modeling, the POF model mice were treated with QWGB by gavage every day and the treatment lasted four weeks. On the 2nd day after the end of the experiment, blood was collected from the eyeballs and the serum was separated by centrifugation. The ovaries and uterus were collected and the adipose tissues were carefully stripped. The organ indexes of the ovaries and uterus of each group were calculated. The serum estrogen(E_2) level of mice in each group was detected by ELISA. Protein samples were extracted from ovarian tissues of mice, and the differential proteins before and after QWGB intervention and before and after modeling were analyzed by quantitative proteomics using tandem mass tags(TMT). As revealed by the analysis of differential proteins, QWGB could regulate 26 differentially expressed proteins related to the POF model induced by T. wilfordii glycosides, including S100A4, STAR, adrenodoxin oxidoreductase, XAF1, and PBXIP1. GO enrichment results showed that the 26 differential proteins were mainly enriched in biological processes and cellular components. The results of KEGG enrichment showed that those differential proteins were involved in signaling pathways such as completion and coalescence cascades, focal adhesion, arginine biosynthesis, and terpenoid backbone biosynthesis. The complement and coalescence cascades signaling pathway was presumably the target pathway of QWGB in the treatment of POF. In this study, the proteomics technique was used to screen the differential proteins of QWGB in the treatment of POF in mice induced by T. wilfordii glycosides, and they were mainly involved in immune regulation, apoptosis regulation, complement and coagulation cascade reactions, cholesterol metabolism, and steroid hormone production, which may be the main mechanisms of QWGB in the treatment of POF.
