Material basis and mechanism of Curcuma longa tuberous roots with and without vinegar processing in treating primary dysmenorrhea.
10.19540/j.cnki.cjcmm.20221101.302
- Author:
Ying PENG
1
;
Bao-Hua DONG
1
;
Yun-Xiu JIANG
1
;
Jie WU
1
;
Ma-Yi-Jie CAO
1
;
Chang-Jiang HU
2
;
Run-Chun XU
1
;
Zhi-Min CHEN
1
Author Information
1. School of Pharmacy,Chengdu University of Traditional Chinese Medicine Chengdu 611137,China State Key Laboratory of Southwestern Chinese Medicine Resources,Chengdu University of Traditional Chinese Medicine Chengdu 611137,China Sichuan Intangible Cultural Heritage Protection and Inheritance Base,Chengdu University of Traditional Chinese Medicine Chengdu 611137,China.
2. School of Pharmacy,Chengdu University of Traditional Chinese Medicine Chengdu 611137,China State Key Laboratory of Southwestern Chinese Medicine Resources,Chengdu University of Traditional Chinese Medicine Chengdu 611137,China Sichuan Intangible Cultural Heritage Protection and Inheritance Base,Chengdu University of Traditional Chinese Medicine Chengdu 611137,China Key Laboratory of Chinese Medicine Formulations Particle Mass and Clinical Evaluation Chengdu 611137,China.
- Publication Type:Journal Article
- Keywords:
Curcuma longa tuberous roots;
molecular docking;
network pharmacology;
primary dysmenorrhea;
processing with vinegar
- MeSH:
Female;
Humans;
Animals;
Rats;
Acetic Acid;
Curcuma;
Dysmenorrhea;
Molecular Docking Simulation;
Tumor Necrosis Factor-alpha;
Cyclooxygenase 2
- From:
China Journal of Chinese Materia Medica
2023;48(3):649-659
- CountryChina
- Language:Chinese
-
Abstract:
Liquid chromatography-mass spectrometry was employed to analyze the chemical components in Curcuma longa tuberous roots(HSYJ), C. longa tuberous roots processed with vinegar(CHSYJ), and rat serum after the administration. The active components of HSYJ and CHSYJ absorbed in serum were identified based on the secondary spectrum of database and literature. The targets of primary dysmenorrhea was screened out from database. The protein-protein interaction network analysis, gene ontology(GO) functional annotation, and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway enrichment analysis were performed for the common targets shared by the drug active components in serum and primary dysmenorrhea, and the component-target-pathway network was constructed. AutoDock was used to conduct molecular docking between the core components and targets. A total of 44 chemical components were identified from HSYJ and CHSYJ, including 18 absorbed in serum. On the basis of network pharmacology, we identified 8 core components(including procurcumenol, isobutyl p-hydroxybenzoate, ferulic acid, and zedoarondiol) and 10 core targets \[including interleukin-6(IL-6), estrogen receptor 1(ESR1), and prostaglandin-endoperoxide synthase 2(PTGS2)\]. The core targets were mainly distributed in the heart, liver, uterus, and smooth muscle. The molecular docking results showed that the core components were well bound to the core targets, indicating that HSYJ and CHSYJ may exert therapeutic effect on primary dysmenorrhea via estrogen, ovarian steroidogenesis, tumor necrosis factor(TNF), hypoxia-inducible factor-1(HIF-1), IL-17 and other signaling pathways. This study clarifies the HSYJ and CHSYJ components absorbed in serum, as well as the corresponding mechanism, providing a reference for further elucidating the therapeutic material basis and clinical application of HSYJ and CHSYJ.
