Expression of hepatitis C virus nonstructural 4B in transgenic mice.
- Author:
Ai Guo WANG
1
;
Hyung Bae MOON
;
Jin Man KIM
;
Soon Bong HWANG
;
Dae Yeul YU
;
Dong Seok LEE
Author Information
1. Laboratory of Human Genomics, Korea Research Institute of Bioscience, and Biotechnology (KRIBB), Daejeon 305-806, Korea. lee10@kribb.re.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
hepacivirus;
liver;
mice, transgenic;
NS4 protein, hepatitis C virus;
viral nonstructural proteins
- MeSH:
Viral Nonstructural Proteins/genetics/*metabolism;
Reverse Transcriptase Polymerase Chain Reaction;
Mice, Transgenic;
Mice, Inbred C57BL;
Mice;
Male;
Liver/metabolism/pathology;
Immunohistochemistry;
Hepacivirus/genetics/*metabolism;
Gene Expression/genetics;
Female;
Blotting, Western;
Animals
- From:Experimental & Molecular Medicine
2006;38(3):241-246
- CountryRepublic of Korea
- Language:English
-
Abstract:
Hepatitis C virus (HCV) is a pathogen that is of great medical significance in chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma worldwide. Although the HCV proteins have been intensively investigated over the past decade, the biochemical functions of the NS4B protein are still largely unknown. To investigate NS4B as a potential causative agent of liver disease, transgenic mice expressing the NS4B protein in liver tissue were produced. The transgenic animals were phenotypically similar to their normal littermates for up to 18 months of age. Our results suggest that the HCV NS4B protein is not directly cytopathic or oncogenic in our transgenic mice model.
