Research on hypoglycemic activity of Osmanthus fragrans var. thunbergii extract
10.16438/j.0513-4870.2022-1071
- VernacularTitle:基于HPLC-ESI-QTOF-MS技术的金桂花降糖活性物质基础研究
- Author:
Jun TONG
1
;
Hong-bing LIU
2
;
Ying-wei LIU
1
;
Fan ZHANG
1
;
Li-juan SUN
1
;
Yong CHEN
1
Author Information
1. National & Local Joint Engineering Research Center of High-throughput Drug Screening Technology, Key Laboratory of Biotechnology of Chinese Traditional Medicine, Health Science and Engineering, Hubei University, Wuhan 430062, China
2. State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan, Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences, Wuhan 430071, China
- Publication Type:Research Article
- Keywords:
italic>Osmanthus fragrans var. thunbergii;
anti-hyperglycemic;
italic>α-glucosidase;
anti-glycosylation;
anti-oxidant
- From:
Acta Pharmaceutica Sinica
2023;58(3):750-759
- CountryChina
- Language:Chinese
-
Abstract:
This study aimed to assess the hypoglycemic activity, and in vitro inhibition of α-glucosidase, inhibition of the advanced glycation end products (AGEs), and total antioxidant capacity were used to clarify its bioactivity. Furthermore, the potential hypoglycemic active chemical constituents in the aqueous extract of Osmanthus fragrans var. thunbergii flower were characterized using high performance liquid chromatography-electrospray ionization-quadruple time-of-flight mass spectrometry (HPLC-ESI-QTOF-MS) method. The result showed that in vitro inhibition of α-glucosidase of the extract (IC50 = 2.11 ± 0.26 mg·mL-1) were similar to acarbose (IC50 = 2.88 ± 0.32 mg·mL-1), and it inhibited the AGEs formation and the total antioxidant capacity in a certain extent. Based on the MS fragmentation pathway analysis of reference chemical acteoside contained in this extract, and related references, 73 constituents were tentatively identified from the aqueous extract of Osmanthus fragrans var. thunbergii flower, including 58 phenylethanoids, 8 caffeoylquinic acids, 1 flavonoid vicenin-2, and 6 common organic chemicals in plant. Furthermore, 8 unknown alkaloids were characterized in this work. Among of these chemicals, 61 phenylethanoids were supposed to be detected for the first time. In conclusion, this work disclosed the potential hypoglycemic active constituents of Osmanthus fragrans var. thunbergii flower.
