Determination of 7 aminoglycoside antibiotics residues in bear bile powder by mix-mode SPE-hydrophilic interaction liquid chromatography tandem mass spectrometry
10.16438/j.0513-4870.2022-0620
- VernacularTitle:混合型固相萃取-亲水作用色谱串联质谱法测定熊胆粉中7种氨基糖苷类抗生素残留量
- Author:
Qing HU
1
;
Jian SUN
2
;
Hong YU
2
;
Jing-xian ZHANG
2
;
Xiu-hong MAO
2
;
Shen JI
1
Author Information
1. China State Institute of Pharmaceutical Industry, Shanghai 201203, China; NMPA Key Laboratory for Quality Control of Traditional Chinese Medicine, Shanghai Institute for Food and Drug Control, Shanghai 201203, China
2. NMPA Key Laboratory for Quality Control of Traditional Chinese Medicine, Shanghai Institute for Food and Drug Control, Shanghai 201203, China
- Publication Type:Research Article
- Keywords:
hydrophilic interaction liquid chromatography tandem mass spectrometry;
mix-mode solid-phase extraction;
bear bile powder;
aminoglycoside
- From:
Acta Pharmaceutica Sinica
2022;57(12):3621-3625
- CountryChina
- Language:Chinese
-
Abstract:
An analytical method was developed for determination of 7 aminoglycosides antibiotics in bear bile powder by hydrophilic interaction liquid chromatography tandem mass spectrometry. The samples were purified by mix-mode weak cation exchange and reversed-phase SPE. Waters ACQUITY UPLC BEH Amide column (100 mm × 3.0 mm, 1.7 μm) was used with 0.2% formic acid aqueous solution-0.2% formic acid acetonitrile solution as mobile phases by gradient elution. The aminoglycosides were detected by electrospray ionization mass spectrometry in positive mode with multiple reaction monitoring (MRM) mode. Spectinomycin, streptomycin, amikacin, kanamycin, tobramycin, apramycin and neomycin possessed good linear correlation in the respective concentration ranges, with the correlation coefficients more than 0.99. The mean recoveries at 3 spiked levels were in the range of 61.3%～127.3%, and the RSDs were 0.1%～1.9%. The limits of quantification were 0.2～1.0 mg·kg-1. The method had been applied to the analysis of actual samples.
