Effects of soluble glycoprotein 130 on expression of p-STAT3 and vascular endothelial growth factor-A in retina of mice with diabetes mellitus
10.3980/j.issn.1672-5123.2023.3.05
- VernacularTitle:sgp130对糖尿病小鼠视网膜p-STAT3及VEGF-A表达的影响
- Author:
Guang-Hui LIU
1
;
Chang-Xuan SHI
1
;
Ya-Jun HONG
1
;
Yong-Zheng ZHENG
1
;
Hang WANG
1
;
Chun MENG
1
Author Information
1. Department of Ophthalmology, Affiliated People's Hospital, Fujian University of Traditional Chinese Medicine, Fuzhou 350004, Fujian Province, China; Eye Institute of Integrated Chinese and Western Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350004, Fujian Province, China
- Publication Type:Journal Article
- Keywords:
diabetic retinopathy;
inflammation;
interleukin 6;
p-STAT3;
vascular endothelial growth factor-A(VEGF-A);
soluble glycoprotein 130(sgp130)
- From:
International Eye Science
2023;23(3):375-378
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To observe the effect of soluble glycoprotein 130(sgp130)on expression of p-STAT3 and vascular endothelial growth factor(VEGF)-A in retina of mice with diabetes mellitus(DM), and explore the possibility of sgp130 in interfering with inflammatory damage of diabetic retinopathy(DR).METHODS: A total of 45 mice were randomly divided into normal group, DM group and sgp130 group. DM models were made in DM group and sgp130 group with streptozotocin. No special intervention was given to normal group and DM group, but sgp130 group was given intravitreal injection of 1.5mg/mL sgp130 2μL at the 1 and 5wk. After 10wk, all the mice were sacrificed to assess the protein expression of interleukin 6(IL-6), p-STAT3 and VEGF-A in the retina.RESULTS: The expressions of IL-6, p-STAT3 and VEGF-A in retina of DM group were higher than those of normal group at 10wk(all P<0.01). The expression of p-STAT3 and VEGF-A in sgp130 group were lower than those in DM group(all P<0.01).CONCLUSION: The sgp130 can selectively antagonize the trans signal transduction pathway of IL-6, down-regulate the expression of downstream inflammatory factors VEGF-A, and it may be used in the intervention of retinal inflammatory damage related with IL-6 in DM.
