A short-term analysis of the modification process of the plaque microbial community before and after treatment in children with S-ECC
10.12016/j.issn.2096-1456.2023.04.007
- Author:
ZHOU Qingnan
1
;
ZHAO Huanying
2
;
CAI Shuang
1
;
YANG Ningyan
1
;
ZHOU Yan
1
;
SHANG Jiajian
1
Author Information
1. Department of Pediatric Dentistry, Capital Medical University
2. Genomics Research Platform Core Facilities Center, Capital Medical University
- Publication Type:Journal Article
- Keywords:
severe early childhood caries;
dental general anesthesia;
oral microecology;
plaque microbial community;
microbiome diversity;
dysbiosis;
biofilm;
caries management
- From:
Journal of Prevention and Treatment for Stomatological Diseases
2023;31(4):267-273
- CountryChina
- Language:Chinese
-
Abstract:
Objective : To explore from the perspective of microorganisms the changes in plaque microbial community of children with severe early childhood caries (S-ECC) before and 3 months after dental treatment. Meanwhile to show the effect of treatment on the maintenance of long- term caries-free state.
Methods: S-ECC children completed dental treatment under general anesthesia. We collected plaque from caries-free dental surfaces before treatment (caries, C) and at the postoperative follow-up review time points of 7 days (C-7D), 1 month (C-1 M), and 3 months (C-3 M). We included caries-free children (caries free, CF) as the control group to analyze the dynamic modification process of the plaque microbial community in the short-term pre- and postdental treatment.
Results:Species clustering analysis showed that the compositions of the microbial communities of the S-ECC and CF groups were highly similar. The α diversity index was not statistically significant (P>0.05). From the analysis of the relative abundance, Leptotrichia spp. and Aggregatibacter spp. decreased after treatment compared with before treatment (P<0.05). Streptococcus sanguinis in the C-7D group increased compared with that in the C group and gradually decreased within 3 months. Veillonella spp., Actinomyces spp., Allprevotella spp., Capnocytophaga spp., and Streptococcus mutans differed between the C and CF groups (P<0.05), Streptococcus mutans did not differ significantly between the C-7D and C-1 M groups and the CF group after treatment, while C-3 M showed an increase compared with the CF group (P<0.01).
Conclusion:The rapid change in the structure of the flora of children with S-ECC after treatment. The plaque microbial community structure in a caries-free state gradually starts to be established 1-3 months after treatment. There is a "core microbiota" in the oral plaque community that jointly maintains microecological stability. Veillonella spp., Allprevotella spp. and Streptococcus mutans have potential as possible microbial markers.
- Full text:S-ECC患儿经治疗后菌斑微生物群落短期内改建过程分析.pdf
