Brazilin downregulates the expression of protein regulator of cytokinesis 1 in bladder cancer T24 cell and BIU87 cell and inhibits the cell proliferation
10.3760/cma.j.cn115355-20211105-00497
- VernacularTitle:巴西苏木素下调膀胱癌T24细胞和BIU87细胞细胞质分裂调控蛋白1的表达及抑制细胞增殖的研究
- Author:
Xihua YANG
1
;
Lili ZHAO
;
Lixia CHEN
;
Yongming YANG
;
Jing WANG
;
Lei YAN
;
Xuebing HAN
Author Information
1. 山西省肿瘤医院 中国医学科学院肿瘤医院山西医院 山西医科大学附属肿瘤医院实验动物中心，太原 030013
- Keywords:
Urinary bladder neoplams;
Brazilin;
Protein regulator of cytokinesis 1;
Proteomics;
Cell proliferation
- From:
Cancer Research and Clinic
2022;34(9):661-664
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the inhibitory effect of brazilin on bladder cancer cells and its mechanism.Methods:Chemically synthesized brazilin was synthesized by chemical synthesis. Methyl thiazolyl tetrazolium (MTT) method was used to detect the inhibitory effect of synthetic brazilin on bladder cancer cells T24 and BIU87. Proteomic technique was used to detect the effect of brazilin on the level of protein in both cells. Real-time quantitative polymerase chain reaction (qRT-PCR) and Western blot methods were used to verify the effects of brazilin on the expression of protein regulator of cytokinesis 1 (PRC1) of both cells at gene and protein level.Results:MTT method showed that brazilin significantly inhibited the proliferation of bladder cancer cells T24 and BIU87, and its half inhibitory concentration ( IC50) of T24 cell and BIU87 cell was 9.9 μg/ml and 5.1 μg/ml,respectively. Proteomic results showed that brazilin could regulate the protein expression of PRC1 in both cells, which was verified by qRT-PCR and Western blot. Conclusion:Brazilin suppresses bladder cancer cell growth possibly by downregulating PRC1.
