Effect of lncRNA SNHG6 targeting miR-485-3p on proliferation and radiotherapy sensitivity of cervical cancer SiHa cells
10.3760/cma.j.cn113030-20210714-00264
- VernacularTitle:lncRNA SNHG6靶向miR-485-3p对宫颈癌SiHa细胞增殖及放疗敏感性的影响
- Author:
Dongli ZHANG
1
;
Guixia SUN
;
Jun TIAN
;
Juncai LIU
Author Information
1. 河南大学淮河医院妇科，开封 475000
- Keywords:
LncRNA SNHG6;
MiR-485-3p;
Uterine cervical neoplasms;
Proliferation;
Radiosensitivity
- From:
Chinese Journal of Radiation Oncology
2022;31(9):828-833
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of lncRNA SNHG6 on the proliferation and radiotherapy sensitivity of cervical cancer SiHa cells and its potential mechanism.Methods:The expression levels of lncRNA SNHG6 and miR-485-3p in cervical cancer tissues, paracancer tissues, SiHa cells and SiHa cells exposed to X-ray were detected. The relationship between lncRNA SNHG6 and miR-485-3p was analyzed. After overexpression or knockdown of SNHG6 and miR-485-3p, cell proliferation ability, number of invasion and apoptosis rate were determined by MTT, Transwell chamber assay and flow cytometry, respectively. The effect of miR-485-3p on the Wnt/β-catenin pathway and the effect of XAV939 on SiHa cell proliferation and radiation sensitivity were analyzed.Results:lncRNA SNHG6 was highly expressed in cervical cancer tissues and SiHa cells, whereas was lowly expressed in X-ray irradiated SiHa cells. miR-485-3p was lowly expressed in cervical cancer tissues and SiHa cells, whereas was highly expressed in X-ray irradiated SiHa cells. lncRNA SNHG6 targeted miR-485-3p. Down-regulation of lncRNA SNHG6 expression inhibited cell proliferation and invasion, and enhanced its sensitivity to X-ray radiotherapy, while miR-485-3p inhibitor transfected cells exerted the opposite effect. The up-regulation of lncRNA SNHG6 promoted the proliferation and invasion of SiHa cells through miR-485-3p, and reduced the sensitivity of radiotherapy. Down-regulation of miR-485-3p activated the Wnt/β-catenin pathway, promoted cell proliferation and invasion of SiHa, and reduced its radiation sensitivity to X-ray.Conclusion:Overexpression of lncRNA SNHG6 targeting miR-485-3p activates the Wnt/β-catenin pathway to regulate the proliferation and radiotherapy sensitivity of SiHa cells.
