Molecular characteristics of Streptococcus suis type 2 from humans in Zhejiang Province
10.3760/cma.j.cn311365-20211105-00386
- VernacularTitle:浙江省猪链球菌2型人源分离株分子分型特征
- Author:
Shuirong ZHU
1
;
Jiancai CHEN
;
Zhuoying WU
;
Yunyi ZHANG
;
Lei FANG
;
Yanjun ZHANG
Author Information
1. 浙江省疾病预防控制中心微生物检验所，杭州 310051
- Keywords:
Streptococcus suis;
Multilocus sequence typing;
Sporadic human isolates;
Molecular typing;
Electrophoresis, gel, pulsed-field;
Minimum core genome
- From:
Chinese Journal of Infectious Diseases
2022;40(8):483-489
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To determine the molecular characteristics of Streptococcus suis type 2 (SS2) in Zhejiang Province. Methods:Twenty-nine SS2 sporadic human isolates in Zhejiang Province from Januery 2005 to July 2021 were genotyped by pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), and minimum core genome (MCG) sequence typing.Results:Among 29 strains, 10 PFGE patterns and three main clusters were obtained by PFGE. Twenty-one (72.41%) of the strains were divided into two main branch groups and the remaining eight (27.59%) showed genetic diversity with the similarity ranging from 49.7% to 94.7%. Three sequence types were obtained from 29 strains by MLST, including ST7 (86.21%(25/29)), ST1 (10.34%(3/29)) and ST25 (3.45%(1/29)). In addition, three genotypes were obtained from 29 strains by MCG, including genotype E (41.38%(12/29)), genotype group 1 (55.17%(16/29)) and genotype group 4 (3.45%(1/29)).Conclusions:Two large clonal groups of highly pathogenic strains of SS2 have been prevalent in Zhejiang Province. A few strains display genetic diversity, indicating genetic variation may exist during transmission.
