Effects of miR-5011-5p on apoptosis and migration of bladder cancer cell line J82 and the underlying mechanism
10.3760/cma.j.cn341190-20210407-00431
- VernacularTitle:miR-5011-5p对膀胱癌细胞系J82凋亡和迁移的影响及机制研究
- Author:
Lei WANG
1
;
Geng HUANG
;
Zhihua YE
;
Weidong JIANG
;
Ni KE
Author Information
1. 鄂东医疗集团黄石市中心医院 湖北理工学院附属医院超声影像科,黄石 435000
- Keywords:
Urinary bladder neoplasms;
Ribonucleases;
Non-coding RNA;
MicroRNA;
Tumor metastasis;
Cell apoptosis;
Cell migration;
Tumor cells,cultured
- From:
Chinese Journal of Primary Medicine and Pharmacy
2022;29(9):1345-1348
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of miR-5011-5p on apoptosis and migration of bladder cancer cell line J82 and the underlying mechanism.Methods:J82 cells were transfected with random sequence molecules (NC group) and miR-5011-5p sequence molecules (miR-5011-5p group). Flow cytometry and scratch experiment were performed to analyze the effects of miR-5011-5p on apoptosis and migration of J82 cells. The target gene of miR-5011-5p was predicted by bioinformatics. Real-time fluorescent quantitative polymerase chain reaction and western blot assay were performed to investigate the effects of miR-5011-5p on target gene expression.Results:The relative expression of miR-5011-5p in J82 cells in the miR-5011-5p group was significantly higher than that in the NC group (10.73 ± 1.67 vs. 1.04 ± 0.16, t = 5.81, P < 0.01). There was significant difference in the apoptosis rate of J82 cells between NC and miR-5011-5p groups [(8.83 ± 1.67)% vs. (34.96 ± 3.80)%, t = 6.30, P < 0.01]. The migration rate of J82 cells differed significantly between NC and miR-5011-5p groups [(71.31 ± 7.69)% vs. (37.43 ± 5.01)%, t = 3.69, P < 0.05]. The target gene of miR-5011-5p may be Yes-related protein 1 (YAP1). Compared with the NC group, miR-5011-5p exhibited an obvious inhibitory effect on the YAP1 expression in J82 cells ( P < 0.01). Conclusion:miR-5011-5p may promote the apoptosis of J82 cells and inhibit their migration in bladder cancer through targeted inhibition of YAP1 gene expression.
