Gene mutation types and clinical characteristics of children with G6PD deficiency in Zunyi area
10.3760/cma.j.cn231583-20210806-00256
- VernacularTitle:遵义地区G6PD缺乏症患儿基因突变类型及临床特点分析
- Author:
Jiang YU
1
;
Tao ZHANG
;
Yuying WANG
;
Fengqin LIN
;
Juan CHEN
;
Hui CHEN
;
Limei YU
Author Information
1. 遵义医科大学附属医院贵州省细胞工程重点实验室 遵义医科大学附属医院贵州省产前诊断分中心 遵义医科大学附属医院遵义市精准医学检测与出生缺陷防控工程研究中心，遵义 563003
- Keywords:
Glucose-6-phosphate dehydrogenase deficiency;
Gene;
Mutation;
Screening
- From:
Chinese Journal of Endemiology
2022;41(8):639-643
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the types of pathogenic gene mutations and their main clinical characteristics in children with glucose-6-phosphate dehydrogenase (G6PD) deficiency in Zunyi area.Methods:Children with clinical manifestations of "yellow staining" or "suspected yellow staining" who were admitted to Guizhou Children's Hospital, Affiliated Hospital of Zunyi Medical University, from September 13, 2018 to September 13, 2020 were selected for G6PD gene mutation detection by multicolor probe melting curve analysis, and the pathogenic gene mutation types and clinical characteristics of children with G6PD deficiency were analyzed.Results:The results of G6PD gene mutation detection showed that among the 1 740 children tested, 119 were positive for gene mutation, and the positive detection rate was 6.84%. The proportion of male infants was higher than that of female infants, and the difference was statistically significant (91 males and 28 females, χ 2 = 15.10, P < 0.001); infancy accounted for 63.87% (76/119), and early childhood accounted for 18.49% (22/119). A total of 11 known pathogenic gene mutation types and 1 unknown mutation were detected. Among the top 4 pathogenic gene mutations, the overall was c.1024 C>T, c.1376 G>T, c.1388 G>A and c.95 A>G, male was c.1376 G>T, c.1388 G>A, c.1024 C>T and c.95 A>G; female was c.1024 C>T, c.95 A>G, c.1388 G>A and c.519 C>T. Among the 119 children with G6PD gene mutation, 90 cases had varying degrees of jaundice, including 36 cases of severe and more severe jaundice (including 2 cases of extremely severe neonatal bilirubin encephalopathy), and 54 cases of mild to moderate jaundice; 37 cases had anemia of different degrees, including 6 cases of mild anemia, 12 cases of moderate anemia, and 19 cases of severe or more severe anemia (including 1 case of extremely severe anemia). Conclusions:There are 12 types of gene mutations in children with G6PD deficiency in Zunyi area, and the most common mutation types are c.1024 C>T, c.1376 G>T, c.1388 G>A and c.95 A>G. Children with G6PD deficiency are often accompanied by varying degrees of jaundice and anemia.
