In situ synthesis of a spherical covalent organic framework as a stationary phase for capillary electrochromatography
- Author:
He NING
1
;
Li ZHENTAO
;
Hu CHANGJUN
;
Chen ZILIN
Author Information
1. Department of Orthopedics Trauma and Microsurgery,Zhongnan Hospital of Wuhan University,School of Pharmaceutical Sciences,Wuhan University,Wuhan,430072,China;Key Laboratory of Combinatorial Biosynthesis and Drug Discovery,Ministry of Education,Hubei Province Engineering and Technology Research Center for Fluorinated Pharmaceuticals,Wuhan,430071,China
- Keywords:
Covalent organic frameworks;
In situ growth method;
Capillary electrochromatography;
Electrochromatographic separation
- From:
Journal of Pharmaceutical Analysis
2022;12(4):610-616
- CountryChina
- Language:Chinese
-
Abstract:
Covalent organic frameworks(COFs)are a novel type of crystalline porous organic polymer materials recently developed.It has several advantages in chromatographic separation field,such as high thermal stability,porosity,structural regularity,and large specific surface area.Here,a novel spherical COF 1,3,5-tris(4-aminophenyl)benzene(TAPB)and 2,5-bis(2-propyn-1-yloxy)-1,4-benzenedicarboxaldehyde(BPTA)was developed as an electrochromatographic stationary phase for capillary electro-chromatography separation.The COFTTAPB-BPTA modified capillary column was fabricated via a facile in situ growth method at room temperature.The characterization results of scanning electron microscopy(SEM),Fourier transform infrared(FT-IR)spectroscopy,and X-ray diffraction(XRD)confirmed that COF TAPB-BPTA were successfully modified onto the capillary inner surface.The electrochromatography separation performance of the COF TAPB-BPTA modified capillary was investigated.The prepared column demonstrated outstanding separation performance toward alkylbenzenes,phenols,and chlorobenzenes compounds.Furthermore,the baseline separations of non-steroidal anti-inflammatory drugs(NSAIDs)and parabens with good efficiency and high resolution were achieved.Also,the prepared column possessed satisfactory precision of the intra-day runs(n=5),inter-day runs(n=3),and parallel columns(n=3),and the relative standard deviations(RSDs)of the retention times of tested alkylbenzenes were all less than 2.58％.Thus,this new COF-based stationary phase shows tremendous application potential in chromatographic separation field.
