Expression of SF3B1, UBE2V2 and SETD2 and association with osteoporotic vertebral fractures inelderly patients
10.3760/cma.j.cn.115807-20211202-00368
- VernacularTitle:SF3B1、UBE2V2和SETD2的表达与老年骨质疏松性椎体骨折的相关性研究
- Author:
Chuanbao WANG
1
;
Xiuli GUO
;
Liyan YU
;
Wenqing QU
;
Shuxia CAI
Author Information
1. 烟台市烟台山医院骨科，烟台 264001
- Keywords:
SF3B1;
UBE2V2;
SETD2;
Osteoporotic vertebral fractures
- From:
Chinese Journal of Endocrine Surgery
2022;16(4):463-467
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the relationship between the expression of SF3B1, UBE2V2, SETD2 and osteoporotic vertebral fracture (OVF) in elderly patients.Methods:Peripheral blood samples were collected from 31 elderly patients with osteoporotic vertebral fractures (VF group) and 16 elderly patients with osteoporotic non-vertebral fractures (NVF group) in Yantai Mountain Hospital. RNA was extracted for transcriptome sequencing to screen for differentially expressed genes. VF related genes were screened by Gene Ontology (GO) analysis, protein protein interaction (PPI) network analysis and ROC curve analysis. Qrt-pcr was used to detect gene expression levels.Results:Compared with NVF group, 691 genes were up-regulated while 131 genes were down regulatedin VF group. qRT-PCR results revealed that, compared with NVF patients (1.55±0.33) (1.70±0.33) (1.64±0.33) , SF3B1 (1.83±0.23) ( t=2.84, P=0.008) , UBE2V2 (2.24±0.43) ( t=3.91, P<0.001) expression were increased while SETD2 (1.18±0.46) ( t=3.25, P=0.003) expression was decreased in peripheral blood of VF patients. ROC curve analysis showed that the AUCs of SF3B1, UBE2V2 and SETD2 in VF were 0.8034 ( P=0.007) , 0.8145 ( P=0.005) and 0.7863 ( P=0.0014) , respectively. Conclusion:SF3B1, UBE2V2 and SETD2 are highly correlated with OVF in elderly patients, and are of great value in the diagnosis and prediction of OVF.
