Discovery and identification of quality markers of Sparganii Rhizoma based on zebrafish thrombosis model
10.1016/j.chmed.2021.04.015
- Author:
Nan XU
1
;
Rong SUN
2
;
Yin-ping SHI
3
;
Hai-yan SHI
3
;
Li-wen HAN
4
Author Information
1. Laboratory of Chinese Medicine Preparation, Shandong Research Academy of Traditional Chinese Medicine
2. Institute of Advanced Medical Sciences, Shandong University
3. Department of Clinical Pharmacy, The First Affiliated Hospital of Shandong First Medical University
4. Institute of Biology of Qilu University of Technology (Shandong Academy of Sciences)
- Publication Type:Journal Article
- Keywords:
anti-thrombotic activity;
Q-Marker;
Sparganii Rhizoma;
zebrafish
- From:
Chinese Herbal Medicines
2021;13(3):389-395
- CountryChina
- Language:Chinese
-
Abstract:
Objective: The aim of the present study was to determine the quality marker (Q-Markers) of Sparganii Rhizoma against thrombus through an integration of investigations on its antithrombotic effect, content determination and spectrum-effect correlation analysis. Methods: Based on the concept of Q-Marker, Sparganii Rhizoma was investigated for the identification of chemical component. The pharmacological effects on arachidonic acid-induced thrombosis in zebrafish were also investigated. The material basis in ethanol extract was determined by HPLC-UV. Furthermore, the potential Q-Markers were analyzed and predicted according to the effect-chemical correlation analysis. Finally, the anti-thrombotic Q-Markers were verified through the anti-thrombotic test of monomer components. Results: The model of thrombosis zebrafish was established with larvae exposed to 100 µmol/L arachidonic acid for 1 h. Nine ingredients in Sparganii Rhizoma were identified as 5-hydroxymethylfurfural, vanillic acid, ferulic acid, p-hydroxybenzaldehyde, p-hydroxybenzoic acid, vanillin, protocatechuic acid, p-coumaric acid and isoferulic acid. According to the determination effect of zebrafish thrombosis model and HPLC content analysis results, all the other contents present positive correlation except 5-hydroxymethylfurfural, and the P values of three representative potential Q-Markers (ferulic acid, protocatechuic acid and p-coumaric acid) were 0.002, 0.001 and 0.026, respectively. Conclusion: Sparganii Rhizoma showed a dose-dependent effect on the recovery of reducing cardiac red blood cell on zebrafish model. Three phenolic acids (ferulic acid, protocatechuic acid and p-coumaric acid) were proved to possess the anti-thrombotic effects which could be regarded as the potential Q-Markers for quality assessment of Sparganii Rhizoma.
