Quantitative determination of multi-class bioactive constituents for quality control of Yiqi Jiangzhi Granules
10.1016/j.chmed.2022.03.001
- Author:
Shaobo GUO
1
;
Shaowei HU
1
;
Lijuan JIANG
1
;
Xiaohe CHEN
1
;
Wei ZHANG
1
;
Yanyan JIANG
1
;
Bin LIU
1
Author Information
1. School of Chinese Materia Medica, Beijing University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
multiple reaction monitoring;
quality control;
traditional Chinese medicine;
UPLC-ESI-MS/MS;
Yiqi Jiangzhi Granules
- From:
Chinese Herbal Medicines
2022;14(2):324-331
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a reliable and sensitive method for evaluating quality of Yiqi Jiangzhi Granules (YQJZG). Methods: Ultra performance liquid chromatography electrospray ionization tandem mass spectrometry (UPLC-ESI-MS/MS) was employed for simultaneous determination of eight marker components. Separation was performed on an AQUITY UPLC® HSS T3 column, the mobile phase consisted of acetonitrile as the organic phase and 0.1% (volume percentage) formic acid as the aqueous. Eight marker components, ginsenoside Rg1 (GRg1), ginsenoside Re (GRe), ginsenoside Rb1 (Gb1), typhaneoside (TEO), isorhamnetin-3-O-neohespeidoside (IN), hesperidin (HPD), aurantio-obtusin-6-O-β-D-glucoside (AG) and curcumin (CCM), were detected by multiple reaction monitoring (MRM) mode. The Chinese Pharmacopoeia (2020 edition) was regarded as the guidance document for this method validation. Results: The method showed good linearity (R
