Expression of miR-342-3p in rheumatoid arthritis patients and its effect on synovial fibroblast inflammation and migration.
10.12122/j.issn.1673-4254.2022.11.16
- Author:
Qin ZHOU
1
;
Jian LIU
1
;
Yan Qiu SUN
1
;
Xiao Lu CHEN
1
;
Xian Heng ZHANG
1
;
Xiang DING
1
Author Information
1. First Affiliated Hospital of Anhui University of Traditional Chinese Medicine, Hefei 230012, China.
- Publication Type:Journal Article
- Keywords:
inflammation;
miR-342-3p;
migration;
rheumatoid arthritis
- MeSH:
Humans;
Anti-Citrullinated Protein Antibodies;
Interleukin-10;
Tumor Necrosis Factor-alpha;
C-Reactive Protein;
Leukocytes, Mononuclear;
Interleukin-6;
Arthritis, Rheumatoid;
Inflammation;
Fibroblasts;
MicroRNAs/genetics*
- From:
Journal of Southern Medical University
2022;42(11):1712-1719
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the expression level of miR-342-3p in peripheral blood mononuclear cells (PBMCs) of patients with rheumatoid arthritis (RA) and its effect on inflammatory response and migration of synovial fibroblasts in RA (RA-FLS).
METHODS:PBMCs were collected from 30 healthy individuals and 50 RA patients for detecting the expression of miR-342-3p, and its correlation with the clinical indicators RF, ESR, anti-CCP, hs-CRP, C3, DAS-28, SAS, and SDS was analyzed. In RA-FLS cultures, the effect of transfection of miR-342-3p mimics and inhibitor on TNF-α-induced inflammatory response of the cells was evaluated by detecting the expressions of IL-1β, IL-6, IL-10, and TNF-α using ELISA. CCK8 assay and Transwell assay were used for detecting the changes in cell viability and migration ability of the synovial cells.
RESULTS:In RA patients, the expression level of miR-342-3p was significantly lowered in the PBMCs (P < 0.05) with an area under the ROC curve of 97.53% and showed inverse correlations with RF (r=-0.321), ESR(r=-0.284), anti-CCP (r=-0.355), hs-CRP (r=-0.320), C3 (r=-0.294), DAS-28 (r=-0.395), SAS (r=-0.366), and SDS (r=-0.397) (all P < 0.05); a low expression of miR-342-3p was strongly associated with elevated levels of anti-CCP, DAS-28, SDS, and SAS (all with a rule support greater than 85%, confidence greater than 88%, and lift greater than 1). In cultured RA-FLS, TNF-α stimulation significantly increased the cell viability (P < 0.05), upregulated the expressions of IL-1β, IL-6, and TNF-α, and lowered the expression of IL-10 (P < 0.05). These changes were significantly suppressed by transfection of the cells with miR-342-3p mimics (P < 0.05) but enhanced by transfection with miR-342-3p inhibitor (P < 0.05).
CONCLUSION:The expression of miR-342-3p is decreased in the PBMCs of RA patients. A lowered expression of miR-342-3p contributes to the pathogenesis of RA by promoting inflammatory responses and migration of RA-FLS.
