The protective effects of diallyl sulfide (DAS) on genotoxicity induced by benzene.
10.3760/cma.j.cn121094-20210106-00005
- VernacularTitle:二烯丙基一硫拮抗苯诱导大鼠遗传毒性损伤
- Author:
Ting YU
1
;
Xiang Xin LI
1
;
Ren Qiang CHEN
1
Author Information
1. Division of Occupational Health and Rodiation Protection, Heze Center for Disease Control and Prevention, Heze 274000, China.
- Publication Type:Journal Article
- Keywords:
Benzene;
Bone-marrow cells;
DNA damage;
Diallyl sulfide;
Flow cytometry;
Lymphocyte
- MeSH:
4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid/analogs & derivatives*;
Allyl Compounds/pharmacology*;
Animals;
Benzene/toxicity*;
Corn Oil;
DNA Damage;
Male;
Rats;
Sulfides/pharmacology*
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2022;40(8):568-572
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the protective effect of diallyl sulfide (DAS) , against benzene-induced genetic damage in rat. Methods: In September 2018, Sixty adult male adaptive feeding 5 days, were randomly divided into six groups according to their weight. Control groups, DAS control groups, benzene model groups, benzene+low DAS groups, benzene+middle DAS groups, benzene+High DAS group, 10 in each group. Rats in the DAS and DAS control group were orally given DAS at 40, 80, 160, 160 mg/kg, blank control and benzene model groups were given corn oil in the same volume. 2 h later, the rats in the benzene model and DAS treatment groups were given gavage administration of benzene (1.3 g/kg) mixed with corn oil (50%, V/V) , blank and DAS control groups were given corn oil in the same volume. Once a day, for 4 weeks. Samples were collected for subsequent testing. Results: Compared with the blank control group, In benzene treated rat, peripheral WBC count was reduced 65.06% (P=0.003) , lymphocyte ratiowas reduced (P=0.000) , micronucleus rate was increased (P=0.000) , Mean fluorescent intensity and relative fluorescence intensity of γH2AX in BMCs were increased 32.69%、32.64% (P=0.001、0.008) , Mean fluorescent intensity and relative fluorescence intensity of γH2AX in PBLs were increased 397.70%、396.26% (P=0.000、P=0.003) respectively. Compared with the benzene model group, the WBC count increased respectively (P=0.000、0.003、0.006) and the micronucleus rate decreased (P=0.000、0.000、0.000) in the DAS groups, Mean fluorescent intensity and relative fluorescence intensity ofγH2AX in BMCs were significantly reduced in the high DAS groups (P=0.000、0.000) , Mean fluorescent intensity and relative fluorescence intensity ofγH2AX in PBLs were significantly reduced in the low, middle, high DAS groups (P=0.000、0.000) . Conclusion: DAS can effectively suppress benzene induced genotoxic damage in rats.
