Effect of Polydatin on Growth，Apoptosis， and ROS/p38 MAPK Signaling Pathway of Myeloma Cells
10.13422/j.cnki.syfjx.20230393
- VernacularTitle:虎杖苷对骨髓瘤细胞的生长、凋亡及ROS/p38 MAPK信号通路作用
- Author:
Yu BAI
1
;
Wenqi XIONG
1
;
Chunlong ZHU
1
;
Xiaoqing LI
1
Author Information
1. The Third Hospital of Nanchang ，Nanchang 330009，China
- Publication Type:Journal Article
- Keywords:
polydatin;
multiple myeloma cells;
growth;
apoptosis;
reactive oxygen species（ROS）/p38 mitogen-activated protein kinase（MAPK） signaling pathway
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2023;29(3):104-109
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo analyze the effects of polydatin on myeloma cell growth，apoptosis， and reactive oxygen species（ROS）/p38 mitogen-activated protein kinase（MAPK） signaling pathway. MethodHuman multiple myeloma （MM） cell line U266 cells were cultured in vitro，and the effects of polydatin at 0，20，40，80，160，200 mg·L-1 on the growth of U266 cells were detected by cell counting kit-8（CCK-8）assay. The half-maximal inhibitory concentration（IC50）was calculated. U266 cells in the logarithmic growth phase were randomly divided into a control group， low- and high-dose polydatin （80 and 160 mg·L-1） groups， and a bortezomib （75 nmol·L-1） group. After treatment with corresponding drugs，the cell viability of each group was determined by CCK-8 assay. The apoptosis rate of each group was measured by flow cytometry. The levels of inflammatory factors， such as tumor necrosis factor-α（TNF-α），interleukin-1β（IL-1β）， and ROS in each group were measured by enzyme-linked immunosorbent assay （ELISA）. The protein expression levels of apoptosis-related factors， including cysteine aspartate-specific protease-9（Caspase-9），B-cell lymphoma-2-associated X protein（Bax），p38 MAPK，and phosphorylated （p）-p38 MAPK in each group were detected by Western blot. ResultCompared with the results in the control group， polydatin of different concentrations could inhibit the growth of U266 cells （P<0.05），and the effect was potentiated with the increase in the concentration，with IC50 of 156.54 mg·L-1. Compared with the control group，the groups with drug treatment showed blunted cell viability （P<0.05） and increased apoptosis rate，TNF-α，IL-1β，and ROS levels， protein expression levels of Caspase-9， Bax，and p-p38 MAPK/p38 MAPK （P<0.05）. Compared with the low-dose polydatin group， the high-dose polydatin group and the bortezomib group showed improved indicators mentioned above （P<0.05）， and there was no significant difference between the high-dose polydatin group and the bortezomib group. ConclusionPolydatin can activate the ROS/p38 MAPK signaling pathway，promote the expression of inflammatory factors，inhibit the growth of U266 cells，and promote their apoptosis.
