Expression and significance of pigment epithelium-derived factor and vascular endothelial growth factor in lens epithelial cells of diabetic patients with cataract
10.3980/j.issn.1672-5123.2023.1.21
- VernacularTitle:2型糖尿病合并白内障患者晶状体上皮细胞中PEDF和VEGF的表达及意义
- Author:
Ya-Ru HU
1
;
Yue CHEN
1
;
Xue HAN
1
;
Ya-Ru CHEN
1
;
Zhu-Yu MA
1
;
Jian-Feng WANG
1
Author Information
1. Department of Ophthalmology, the First Affiliated Hospital of Bengbu Medical College, Bengbu 233004, Anhui Province, China; Bengbu Medical College, Bengbu 233030, Anhui Province, China
- Publication Type:Journal Article
- Keywords:
diabetes mellitus with cataract;
lens epithelial cells(LECs);
pigment epithelium-derived factor(PEDF);
vascular endothelial growth factor(VEGF)
- From:
International Eye Science
2023;23(1):109-112
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To observe the expression levels of pigment epithelium-derived factor(PEDF)and vascular endothelial growth factor(VEGF)in lens epithelial cells(LECs)of diabetic patients with age-related cataract, and explore the pathogenesis of diabetic with age-related cataract.METHODS: Retrospectively study. A total of 30 patients with age-related cataract(ARC group)and 30 patients with type 2 diabetes mellitus combined with age-related cataract(DC group)who treated at the Department of Ophthalmology of the First Affiliated Hospital of Bengbu Medical College from August 2020 to April 2021 were collected. Anterior capsule specimens with a diameter of 5.5-6.0mm in the central region of the intraoperative eye were collected during phacoemulsification. The protein expression levels of PEDF and VEGF in LECs were detected by Western-blot. Quantitative real-time PCR(qRT-PCR)was used to detect the relative mRNA expression levels of PEDF and VEGF.RESULTS:PEDF and VEGF were expressed in LECs of both groups, and the relative expression of VEGF mRNA in DC group was 1.364±0.062, which was higher than that of ARC group(1.000±0.0; P<0.01). The relative mRNA expression level of PEDF was 0.398±0.053, which was significantly lower than 1.000±0.0 in the ARC group(P<0.001). The expression of VEGF protein in LECs was 2.053±0.026 in DC group and 1.680±0.064 in ARC group, respectively. Meanwhile, the expression of PEDF protein was 0.579±0.045 in DC group and 1.058±0.007 in ARC group(all P<0.01).CONCLUSION:The changes of PEDF and VEGF expression levels in LECs may be related to the occurrence and development of cataract in diabetic patients.
