The Effect of Morphine On Tumor Growth of Melanoma And The Immunotherapy Efficacy
10.13471/j.cnki.j.sun.yat-sen.univ（med.sci）.2022.0609
- VernacularTitle:吗啡对小鼠黑色素瘤的生长及免疫治疗效果的影响
- Author:
Pei-zhang LIANG
1
;
Hao-qian ZHU
1
;
Xiao-min ZHANG
2
;
Hao CHEN
1
;
Ling-zhi WANG
1
Author Information
1. Department of Anesthesiology， The Second Affiliated Hospital of Guangzhou Medical University， Guangzhou 510260，China
2. Sun Yat-sen University cancer center， Guangzhou 510060，China
- Publication Type:Journal Article
- Keywords:
morphine;
CD8+ T cell;
melenoma;
immunotherapy efficacy
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2022;43(6):938-945
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo study the effect of morphine on the function of CD8+ T cells， tumor growth of melanoma and immunotherapy efficacy of PD-1. MethodIsolated CD8+ T cells were activated in vitro and stimulated with different concentrations of morphine. Flow cytometry was used to analyze the percentage of activated CD8+ T cell and the secretion of IFN+. Female C57/BL6 mice were inoculated with mouse melanoma tumor cells. On day 7 after inoculation， middle dose of morphine was injected intraperitoneally and the tumor volume was recorded. The tumor growth curve and survival curve of mice were plotted. Female C57/BL6 mice were inoculated with mouse melanoma tumor cells and were intraperitoneally treated with 200ug PD-1 antibody for 3 times continuously. Meanwhile， middle dose of morphine was injected intraperitoneally and tumor volume was measured. The tumor growth curve and survival curve of the mice were plotted. ResultsCompared with control group ， middle and high doses of morphine significantly inhibited the activation of CD8+ T cells and the secretion of IFN+（P<0.05）； middle doses of morphine also promoted tumor growth （P<0.01） and shortened the survival time of tumor bearing mice. Combined treatment with PD-1 antibody， morphine significantly increased tumor growth（P<0.01） and shortened the survival time of bearing tumor mice. ConclusionsMorphine promote tumor growth and antagonize the efficacy of PD-1 immunotherapy. These effects may be related to the inhibition of morphine on the activation and function of CD8+ T cells.
