Comparison of protein binding rate of main effective components of Houpu paiqi mixture in animal plasma of different species
- VernacularTitle:厚朴排气合剂主要药效成分在不同种属动物血浆中蛋白结合率的比较
- Author:
Mingxin GUO
1
;
Wentao FAN
2
;
Xia WU
3
;
Ying SHEN
2
;
Zhiqiang HU
2
Author Information
1. Dept. of Pharmacy，the Affiliated Yixing Hospital of Jiangsu University，Jiangsu Yixing 214200，China;New Drug Research and Development Center of Guangdong Pharmaceutical University，Guangzhou 510006，China
2. Dept. of Pharmacy，the Affiliated Yixing Hospital of Jiangsu University，Jiangsu Yixing 214200，China
3. New Drug Research and Development Center of Guangdong Pharmaceutical University，Guangzhou 510006，China
- Publication Type:Journal Article
- Keywords:
Houpu paiqi mixture;
magnolol;
honokiol;
plasma protein binding rate;
equilibrium dialysis;
bovine plasma
- From:
China Pharmacy
2022;33(24):2990-2994
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To determine the protein binding rates of main effective components in Houpu paiqi mixture as magnolol and honokiol in animal plasma of different species， and compare the differences among species. METHODS The plasma of bovine， rabbit and rat （internal dialysate） was placed in 4.5， 9.0 and 13.5 μg/mL （according to the weight of the decoction pieces of Houpu paiqi mixture） dialysate containing drug for 24 h by equilibrium dialysate method. The concentrations of magnolol and honokiol in dialysis internal and external fluids were determined by ultra-performance liquid chromatography-tandem triple quadrupole mass spectrometry， and the plasma protein binding rates were calculated and compared. RESULTS At the concentrations of 4.5， 9.0 and 13.5 μg/mL， the protein binding rates of magnolol were （68.13±4.52）%， （74.34±1.12）% and （86.22±0.50）% in bovine plasma， （59.55±4.62） %， （72.81±4.56）% and （86.40±1.91）% in rabbit plasma， and （56.63± 2.87）%， （77.81±1.83）% and （83.18±0.65）% in rat plasma， respectively. The protein binding rates of honokiol were （34.82± 1.67）%， （40.29±3.28）% and （63.57±0.59）% in bovine plasma， （34.25±5.62）%， （62.12±7.36）% and （80.86±4.01）% in rabbit plasma， and （37.06±3.28）% ， （52.61±1.69）% and （79.83±7.38）% in rat plasma， respectively. There was generally statistical significance among those groups （P＜0.05 or P＜0.01）. CONCLUSIONS There are significant species differences in plasma protein binding rates of magnolol and honokiol， the main effective components of Houpu paiqi mixture， in a certain concentration-dependent trend.
