Specific primers design based on the superoxide dismutase b gene for Trypanosoma cruzi as a screening tool: Validation method using strains from Colombia classified according to their discrete typing unit
10.1016/S1995-7645(14)60149-8
- Author:
Francisco OLMO
1
;
Patricia PALMA
1
;
Manuel SÁNCHEZ-MORENO
1
;
Clotilde MARÍN
1
;
Javier ESCOBEDO-ORTEG
2
;
Ana MEJÍA-JARAMILLO
3
;
Omar TRIANA
3
Author Information
1. Grupo de Parasitología Molecular, Departmento de Parasitología; Universidad de Granada
2. Laboratorio de Zoonosis y ETVD's, C.I.R. Dr. Hideyo Noguchi, Universidad Autónoma de Yucatán
3. Grupo Biología y Control de Enfermedades Infecciosas BCEI - SIU, Instituto de Biología, Universidad de Antioquia
- Publication Type:Journal Article
- Keywords:
Colombia;
Discrete typing unit;
Polymerase chain reaction;
Superoxide dismutase gene b - based primers;
Trypanosoma cruzi
- From:
Asian Pacific Journal of Tropical Medicine
2014;7(11):854-859
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To classify 21 new isolates of Trypanosoma cruzi (. T. cruzi) according to the Discrete Typing Unit (DTU) which they belong to, as well as tune up a new pair of primers designed to detect the parasite in biological samples. Methods: Strains were isolated, DNA extracted, and classified by using three Polymerase Chain Reactions (PCR). Subsequently this DNA was used along with other isolates of various biological samples, for a new PCR using primers designed. Finally, the amplified fragments were sequenced. Results: It was observed the predominance of DTU I in Colombia, as well as the specificity of our primers for detection of T. cruzi, while no band was obtained when other species were used. Conclusions: This work reveals the genetic variability of 21 new isolates of T. cruzi in Colombia.Our primers confirmed their specificity for detecting the presence of T. cruzi.
