Effect and mechanism of miR-34a on proliferation, apoptosis and invasion of laryngeal carcinoma cells
10.1016/j.apjtm.2016.03.018
- Author:
Ju-Xiang WANG
1
;
Qing-Jun ZHANG
1
;
Shi-Geng PEI
1
;
Bao-Liang YANG
1
Author Information
1. Affiliated Hospital of Hebei University of Engineering
- Publication Type:Journal Article
- Keywords:
Apoptosis;
Invasion;
Laryngeal squamous carcinoma Hep2 cells;
MiR-34a;
Proliferation
- From:
Asian Pacific Journal of Tropical Medicine
2016;9(5):494-498
- CountryChina
- Language:Chinese
-
Abstract:
Objective To discuss the effect and mechanism of miR-34a on the proliferation, apoptosis and invasion of laryngeal carcinoma cells. Methods The laryngeal squamous carcinoma Hep2 cells were transiently transfected with miR-34a mimics and miR-34a NC. The MTT, colony-forming assay, Hoechst staining and AnnexinV-PI double staining flow cytometry were employed to detect the effect of miR-34a on the viability and apoptosis of laryngeal squamous carcinoma Hep2 cells; Transwell assay to defect the effect of miR-34a on the migration and invasion of laryngeal squamous carcinoma Hep2 cells; western blot and RT-PCR assay to defect the effect of miR-34a mimics on the expression of survivin and Ki-67 mRNA in laryngeal squamous carcinoma Hep2 cells. Results Compared with miR-34a NC group, the cell viability in miR-34 mimics group was significantly decreased (P < 0.01), the cell apoptosis rate was significantly increased (P < 0.01), the abilities of cell migration and invasion were significantly reduced (P < 0.01) and the expression of survivin and Ki-67 mRNA was significantly decreased (P < 0.01). Conclusions The increased expression of miR-34a can induce the apoptosis of Hep2 laryngeal carcinoma cells and inhibit the cell proliferation and invasion, which is related to the down-regulated expression of survivin and Ki-67.
