Phytochemical screening and in vitro evaluation of antioxidant and antimicrobial activities of Kedrostis africana (L.) Cogn
10.1016/j.apjtb.2017.09.008
- Author:
Jeremiah Oshiomame UNUOFIN
1
;
Gloria Aderonke OTUNOLA
1
;
Anthony Jide AFOLAYAN
1
Author Information
1. Medicinal Plants and Economic Development Research Centre, Department of Botany, University of Fort Hare
- Publication Type:Journal Article
- Keywords:
Agar dilution;
Antimicrobial activity;
Radical scavenging activity;
Total flavonoid;
Total phenolics
- From:Asian Pacific Journal of Tropical Biomedicine
2017;7(10):901-908
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate phytochemical, antioxidant and antimicrobial activities of Kedrostis africana (K. africana). Methods Dried tubers of K. africana were extracted in acetone, water and ethanol. The total phenol, flavonoid, proanthocyanidin and tannin contents were determined spectrometrically. The antioxidant activity was examined using 2,2-diphenyl-1-picrylhydrazyl, 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt, nitric oxide and hydrogen peroxide assays. The antimicrobial activity was determined by agar dilution method using minimum inhibitory concentration against 3 g positive and three gram negative strains while four fungal strains were also investigated. Results Total phenol, flavonoids, proanthocyanidin and tannin contents ranged from (5.32 ± 0.01) to (10.51 ± 0.01) mg GAE/g; (42.58 ± 0.02) to (529.23 ± 0.01) mg QE/g; (15.05 ± 0.00) to (585.64 ± 0.00) mg CE/g and (0.301 ± 0.010) to (0.937 ± 0.000) mg TAE/g, respectively. The IC
