Rumex acetosella Inhibits Platelet Function via Impaired MAPK and Phosphoinositide 3-Kinase Signaling.
10.1007/s11655-021-2873-0
- Author:
Bo-Ra JEON
1
;
Muhammad IRFAN
1
;
Seung Eun LEE
2
;
Jeong Hoon LEE
2
;
Man Hee RHEE
3
Author Information
1. Laboratory of Physiology and Cell Signaling, College of Veterinary Medicine, Kyungpook National University, Daegu, 41566, Republic of Korea.
2. Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science (NIHHS), Eumseong, 27709, Republic of Korea.
3. Laboratory of Physiology and Cell Signaling, College of Veterinary Medicine, Kyungpook National University, Daegu, 41566, Republic of Korea. rheemh@knu.ac.kr.
- Publication Type:Journal Article
- Keywords:
Rumex acetosella L;
antiplatelet;
ethnomedicine;
integrin α IIb β 3;
mitogen-activated protein kinase
- MeSH:
Blood Platelets/metabolism*;
Calcium/metabolism*;
Fibrinogen/metabolism*;
Mitogen-Activated Protein Kinases/metabolism*;
Phosphatidylinositol 3-Kinase/pharmacology*;
Phosphatidylinositol 3-Kinases/metabolism*;
Phosphorylation;
Plant Extracts/pharmacology*;
Platelet Aggregation;
Platelet Aggregation Inhibitors/pharmacology*;
Platelet Glycoprotein GPIIb-IIIa Complex/pharmacology*;
Proto-Oncogene Proteins c-akt/metabolism*;
Rumex/metabolism*
- From:
Chinese journal of integrative medicine
2022;28(9):802-808
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:To examine the antiplatelet and antithrombotic activity of Rumex acetosella extract.
METHODS:Standard light aggregometry was used for platelet aggregation, intracellular calcium mobilization assessed using Fura-2/AM, granule secretion (ATP release) by luminometer, and fibrinogen binding to integrin αIIbβ3 detected using flow cytometry. Western blotting is carried out to determine the phosphorylation of mitogen-activated protein kinase (MAPK) and phosphoinositide 3-kinase (PI3K)/Akt signaling.
RESULTS:Rumex acetosella displayed the ability to inhibit platelet aggregation, calcium mobilization, granule secretion, and fibrinogen binding to integrin αIIbβ3. Rumex acetosella has also down-regulated MAPK and PI3K/Akt phosphorylation (all P<0.01).
CONCLUSION:Rumex acetosella extract exhibits antiplatelet activity via modulating GPVI signaling, and it may protect against the development of platelet-related cardiovascular diseases.
