Expression and Significance of PD-1, PD-L1 and CTLA-4 in the Bone Marrow of Patients with Multiple Myeloma.
10.19746/j.cnki.issn.1009-2137.2022.06.027
- Author:
Qing-Zhao LI
1
;
Juan PENG
1
;
Hai-Mei CHEN
1
;
Zhen-Zhen CHEN
1
;
Zhan-Wang ZHU
1
;
Ling-Shu LI
1
;
Guo-Yu HU
1
;
Zhao-Hui YUAN
2
Author Information
1. Department of Hematology, The Affiliated Zhuzhou Hospital, Xiangya Medical College, Central South University, Zhuzhou 412000, Hunan Province, China.
2. Department of Hematology, The Affiliated Zhuzhou Hospital, Xiangya Medical College, Central South University, Zhuzhou 412000, Hunan Province, China,E-mail: 454572267@qq.com.
- Publication Type:Journal Article
- Keywords:
flow cytometry;
multiple myeloma;
programmed death receptor 1;
regulatory T cells
- MeSH:
Humans;
B7-H1 Antigen;
Bone Marrow;
CD8-Positive T-Lymphocytes;
Multiple Myeloma;
Programmed Cell Death 1 Receptor
- From:
Journal of Experimental Hematology
2022;30(6):1803-1809
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the expression and significance of PD-1, PD-L1 and CTLA-4 tumor-associated antigens in multiple myeloma.
METHODS:Bone marrow specimens from 122 patients with multiple myeloma were collected and divided into new-onset group (NDMM), complete remission group (CRMM) and relapsed and refractory group (RRMM) according to the disease progression stage. The proportion of CD4+ T lymphocytes, CD8+ T lymphocytes, Treg cells and plasma cells in the specimens and the expressions of PD-1, PD-L1 and CTLA-4 were detected by multi-parameter flow cytometry.
RESULTS:There was no significant difference in the proportion of CD8+T and Treg cells among the three groups (P>0.05), while the proportions of CD4+T cells and PC in NDMM group were significantly higher than those in the CRMM group (P<0.05), the ratios of CD4+ to CD8+T in the NDMM and RRMM groups were significantly higher than those in the CRMM group (P<0.05). The expressions of PD-1, PD-L1 and CTLA-4 in CD8+ T cells was no significant difference among NDMM, CRMM and RRMM groups (P>0.05). While the expressions of PD-1, PD-L1 and CTLA-4 in CD4+ T cells and PC in the NDMM group were significantly lower than that in the CRMM group (P<0.05). There was significantly difference among the three groups in the expression of PD-1 in Treg cells, of which the NDMM group was significantly lower than that of the CRMM group (all P<0.05). The expressions of PD-1 and CTLA-4 in PC were significantly higher than those in CD8+ T, CD4+ T and Treg cells (P<0.05), the expression of PD-L1 in CD8+ T cells was significantly higher than that in CD4+ T and Treg cells (P<0.05).
CONCLUSION:There is a correlation between the immune status of multiple myeloma and the expressions of PD-1, PD-L1 and CTLA-4 in plasma cells and lymphocyte subsets in vivo.
