Value of metagenomic next-generation sequencing in children with hemophagocytic syndrome with central nervous system involvement.
10.7499/j.issn.1008-8830.2206118
- Author:
Hai-Yang ZHANG
1
;
Mao-Ting TANG
1
;
Lu QING
1
;
De-Yuan LI
1
;
Li-Na QIAO
1
Author Information
1. Department of Pediatric Intensive Care Unit, West China Second University Hospital, Sichuan University/Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, Chengdu 610041, China.
- Publication Type:Journal Article
- Keywords:
Child;
Epstein-Barr virus;
Hemophagocytic syndrome;
Metagenomic next-generation sequencing
- MeSH:
Child;
Humans;
Lymphohistiocytosis, Hemophagocytic/genetics*;
Epstein-Barr Virus Infections/complications*;
Herpesvirus 4, Human/genetics*;
Retrospective Studies;
High-Throughput Nucleotide Sequencing;
Central Nervous System
- From:
Chinese Journal of Contemporary Pediatrics
2022;24(11):1226-1230
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVES:To study the value of metagenomic next-generation sequencing (mNGS) in detecting intracranial Epstein-Barr virus (EBV) infection in children with hemophagocytic syndrome (HPS) with central nervous system involvement.
METHODS:A retrospective analysis was performed for the cerebrospinal fluid mNGS results of 30 HPS children with central nervous system involvement, which were compared with the results of cerebrospinal fluid EBV-DNA detection and serum EBV antibody profile. The change in serum EBV-DNA copy number after treatment was used to evaluate the efficacy of targeted therapy.
RESULTS:The positive rate of EBV in cerebrospinal fluid determined by mNGS was significantly higher than that of EBV-DNA in cerebrospinal fluid (100% vs 10%, P<0.001) and had no significant difference from the positive rate of serum EBV antibody profile (100% vs 93%, P>0.05). The median number of sequences determined by mNGS was 2 400, and serum EBV-DNA copy number before treatment was moderately positively correlated with the number of EBV sequences (rs=0.693, P<0.001). The multiple linear regression analysis showed that the number of sequences determined by mNGS in cerebrospinal fluid increased with the increase in serum EBV-DNA copy number before treatment (P<0.05).
CONCLUSIONS:EBV-associated HPS often results in EBV-infected viral encephalitis, and mNGS can significantly increase the detection rate of EBV in cerebrospinal fluid, which may help with clinical diagnosis.
