Composition and immunomodulatory activity of neutral and acidic polysaccharides isolated from Epimedii Folium.
10.19540/j.cnki.cjcmm.20210525.306
- Author:
Yan-Jun YANG
1
;
Chang LI
2
;
Fei-Fei CHEN
3
;
Mao-Mao ZHU
1
;
Xiao-Bin JIA
1
;
Liang FENG
1
;
Xiao-Bin TAN
3
Author Information
1. School of Traditional Chinese Pharmacy,China Pharmaceutical University Nanjing 211198,China.
2. School of Traditional Chinese Pharmacy,China Pharmaceutical University Nanjing 211198,China Nanjing Integrated Traditional Chinese and Western Medicine Hospital Affiliated to Nanjing University of Chinese Medicine Nanjing 210028, China.
3. Nanjing Integrated Traditional Chinese and Western Medicine Hospital Affiliated to Nanjing University of Chinese Medicine Nanjing 210028, China.
- Publication Type:Journal Article
- Keywords:
Epimedii Folium;
antigen presenting cell;
immunomodulatory activity;
polysaccharides;
structural characterization
- MeSH:
Cytokines/metabolism*;
Drugs, Chinese Herbal;
Gas Chromatography-Mass Spectrometry;
Humans;
Immunomodulation;
Polysaccharides/chemistry*;
Spectroscopy, Fourier Transform Infrared
- From:
China Journal of Chinese Materia Medica
2022;47(16):4358-4364
- CountryChina
- Language:Chinese
-
Abstract:
Epimedii Folium possesses many pharmacological activities including immunomodulation, anti-oxidation, and anti-tumor. Polysaccharides are the main components of Epimedii Folium, and their activities are closely related to the structure. The present study isolated a neutral polysaccharide(EPS-1-1) and an acidic polysaccharide(EPS-2-1) from the aqueous extract of Epimedii Folium through DEAE-52 cellulose anion-exchange chromatography and Sephadex G-100. The structures were characterized by chemical composition analysis, high-performance gel permeation chromatography(HPGPC), Fourier-transform infrared spectrometry(FT-IR), 1-phenyl-3-methyl-5-pyrazolone(PMP) derivatization, scanning electron microscopy(SEM), Congo red test, etc. The immunomodulatory activity of polysaccharides in vitro was determined by investigating the effects on the maturation of bone marrow-derived dendritic cells(BMDCs) and the release of inflammatory cytokines. According to the structural characterization analysis, EPS-1-1 was composed of fructose(Fuc), mannose(Man), ribose(Rib), rhamnose(Rha), glucose(Glc), galactose(Gal), xylose(Xyl), and arabinose(Ara) at 1.90∶0.67∶0.05∶0.08∶3.29∶1.51∶0.05∶0.37(molar ratio), while EPS-2-1 was mainly composed of Fuc, Man, Rha, glucuronic acid(GlcA), galacturonic acid(GalA), Glc, Gal, Xyl, and Ara at 5.25∶0.18∶0.32∶0.13∶1.14∶0.16∶0.55∶0.08∶0.2. EPS-1-1 and EPS-2-1 could promote the maturation and function of BMDCs through up-regulating the expression of MHC-Ⅱ, CD86, CD80, and CD40, and increasing the levels of inflammatory cytokines(IL-6, IL-12, and TNF-α) in vitro experiments, which suggested that EPS-1-1 and EPS-2-1 possessed good immunomodulatory activity.
