BCG-Induced Dendritic Cell Responses and Suppression of Interleukin-5 Production from T Cells in Atopic Asthmatics.
10.3346/jkms.2008.23.4.628
- Author:
Inseon S CHOI
1
;
Xiang Hua LIN
;
Young Ah KOH
;
Yong CUI
Author Information
1. Department of Allergy, Chonnam National University Medical School, Gwangju, Korea. ischoi@chonnam.chonnam.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Asthma;
Atopic;
Mycobacterium Bovis;
Dendritic Cells
- MeSH:
Adult;
Asthma/*immunology;
BCG Vaccine/*immunology;
Dendritic Cells/*immunology;
Female;
Humans;
Interferon-gamma/biosynthesis;
Interleukin-10/biosynthesis;
Interleukin-12/biosynthesis;
Interleukin-5/*biosynthesis;
Lymphocyte Activation;
Male;
T-Lymphocytes/*immunology
- From:Journal of Korean Medical Science
2008;23(4):628-634
- CountryRepublic of Korea
- Language:English
-
Abstract:
Bacille Calmette-Guerin (BCG) induces potent Th1 responses with the help of interleukin (IL)-10 and IL-12 released from dendritic cells (DCs), and suppresses Th2- associated allergic reactions. However, there are still some controversies on therapeutic effects of BCG in asthmatics. This study investigated whether BCG administration to DCs suppresses IL-5 production from T cells in atopic asthmatics. DCs derived from peripheral blood of subjects were cultured with or without BCG and Dermatophagoides farinae extract. Some DCs were co-cultured with T cells in the presence of BCG or the above culture supernatants. In the atopic asthmatics, BCG significantly increased IL-10 and IL-12 production from DCs. In the presence of D. farinae extract, BCG further increased IL-10 production. BCG-induced IL-10 production was significantly higher in the atopics (n=14) than in the non-atopics (n=9). Both BCG and the BCG-treated DCs culture supernatant significantly increased IFN-gamma production from T cells. Both BCG and the supernatant from DCs+BCG+D. farinae co-cultures significantly decreased IL-5 production (all p<0.05), but the supernatant from DCs+BCG co-cultures did not. In conclusion, administration of BCG together with D. farinae extract effectively decreased IL-5 production from T cells, probably through the action of IL-10 and IL-12 released from DCs in D. farinaesensitive asthmatics.
