Protective Effect of Naringin on Alcohol-induced Acute Liver Injury
10.13422/j.cnki.syfjx.20221703
- VernacularTitle:柚皮苷对酒精诱导急性肝损伤的保护作用
- Author:
Shuxia ZHANG
1
;
Xuelian ZHANG
1
;
Shan LU
2
;
Yun LUO
1
;
Xiaobo SUN
1
Author Information
1. Institute of Medicinal Plant Development，Chinese Academy of Medical Sciences，Beijing 100193，China
2. Beijing Increasepharm Corporation Limited，Beijing 102206，China
- Publication Type:Journal Article
- Keywords:
naringin;
alcoholic liver injury;
hepatic enzyme;
hepatocyte apoptosis
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2023;29(1):61-66
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the pharmacodynamic effect of naringin on mice with alcohol-induced liver injury and provide data support for the development of naringin as an anti-alcoholic and liver-protecting drug. MethodSixty Balb/c mice were randomly divided into six groups according to body weight， namely， the control group， the model group， the naringin low and high-dose group （25， 50 mg·kg-1）， Haiwang Jinzun tablet positive control group （2 g·kg-1）， and naloxone positive control group （2 mg·kg-1）. Each group was given corresponding drugs by injection or gavage， and the control group and the model group were given equal volume of 0.5% sodium carboxymethyl cellulose solution by gavage， once a day for 14 consecutive days. Except those in the control group， mice in other groups were additionally given 56° Chinese Baijiu （13 mL·kg-1） for 14 days to induce the mouse model of alcoholic liver injury. One day before the last administration， mice were fasted for 12 h. Eyeballs were removed for blood after the last administration of Chinese Baijiu， and the livers were collected and weighed. The activity levels of alanine transaminases （ALT） and aspartate aminotransferase （AST） were detected by automatic biochemical analyzer. Hematoxylin-eosin （HE） staining was performed to determine and compare the pathological changes in liver tissues of mice， and the ratio of positive cells were observed by TUNEL/DAB dual staining method. Western blot was used to determine the expression of apoptosis-related proteins including B-cell lymphoma-2 （Bcl-2）， Bcl-2 associated X protein （Bax）， and Cytochrome-C （Cyt-C）. ResultAs compared with the control group， the liver/body ratio of the model group was significantly increased （P<0.01）， and the expression of ALT and AST in the serum was obviously increased （P<0.01）. Further， the model group showed severe loosing of hepatocyte cytoplasm， edematous， steatosis， and apoptosis of hepatocytes， with obvious bleeding phenomena. In addition， the apoptosis of liver cells increased， the Bal-2/Bax ratio was decreased （P<0.01）， and the level of pro-apoptotic protein Cyt-C was increased in the model group （P<0.01）. Compared with the model group， the naringin low and high-dose groups reduced the liver/body ratio （P<0.05， P<0.01）， and the high-dose and low-does group significantly inhibited the activity levels of ALT and AST in the serum of mice （P<0.05， P<0.01）. Moreover， the hepatocyte steatosis was significantly reduced， hepatocyte edema disappeared， and bleeding was improved in the naringin high-dose group， and the TUNEL-positive rate was down-regulated. The naringin low and high-dose groups decreased the Bcl-2/Bax ratio （P<0.05， P<0.01） and enhanced the expression level of Cyt-C （P<0.05）. ConclusionNaringin protects alcohol-induced liver damage by regulating the expression levels of ALT and AST in the serum， improving liver fatty lesions， and reducing hepatocyte apoptosis.
