Analysis of essential oil from medicinal and non-medicinal parts of Atractylodes chinensis and antibacterial activity in vitro
- VernacularTitle:北苍术药用和非药用部位挥发油成分分析及体外抗菌活性考察
- Author:
Jianyun ZHANG
1
;
Yanyun ZHAO
1
;
Yunsheng ZHAO
1
;
Zongsheng YAN
1
;
Litao LU
2
;
Yuguang ZHENG
3
;
Huiyong FANG
2
Author Information
1. College of Pharmacy，Hebei University of Chinese Medicine/Hebei Province Processing Technology Innovation Center of Traditional Chinese Medicine，Shijiazhuang 050200，China;International Joint Research Center of Resource Utilization and Quality Evaluation of Traditional Chinese Medicine of Hebei Province，Shijiazhuang 050200，China
2. College of Pharmacy，Hebei University of Chinese Medicine/Hebei Province Processing Technology Innovation Center of Traditional Chinese Medicine，Shijiazhuang 050200，China
3. College of Pharmacy，Hebei University of Chinese Medicine/Hebei Province Processing Technology Innovation Center of Traditional Chinese Medicine，Shijiazhuang 050200，China;International Joint Research Center of Resource Utilization and Quality Evaluation of Traditional Chinese Medicine of Hebei Province，Shijiazhuang 050200，China;Hebei Chemical and Pharmaceutical College，Shijiazhuang 050026，China
- Publication Type:Journal Article
- Keywords:
Atractylodes chinensis;
non-medicinal parts;
medicinal parts;
essential oil;
antibacterial activity;
component
- From:
China Pharmacy
2022;33(21):2609-2614
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To explore the difference in chemical components of essential oil from medicinal part （rhizome）and non-medicinal parts （fibrous roots ，stems，and leaves ）of Atractylodes chinensis，and to preliminarily evaluate their antibacterial activity in vitro . METHODS The essential oil was extracted from three parts of A. chinensis by steam distillation ，and their chemical components and relative contents were analyzed and identified by gas chromatography -mass spectrometry （GC-MS）. The antibacterial activities of essential oil （5 mg/mL） from different parts against Staphylococcus aureus ，S. albus and Bacillus subtilis were determined by paper diffusion method . The correlation between chemical components in essential oil and antibacterial activity was analyzed . RESULTS A total of 60 chemical constituents were identified from the essential oilof rhizome ，fibrous roots ，stems and leaves of A. chinensis， mainly composed of terpenes ，sesquiterpenoids，alcohols and ketones. There were 8 kinds of common components in the essential oil of rhizome ，fibrous root and stem and leaves ，which were elementol ，cadinene，agarospirol，valencian citrine ， caryophyllin，β-sesquidiene，γ-eucalyptol and β-eucalyptol. A total of 26 components were identified from the essential oil of rhizome，of which the relative content of 4-benzaldehyde was the highest （31.56%）；a total of 21 components were identified from the essential oil of fibrous roots ，among which the relative content of β-eucalyptol was the highest （44.39%）；a total of 42 components were identified from the essential oil of stems and leaves ，among which the relative content of β-sesquidiene was the highest（14.83%）. The essential oil from different parts had certain antibacterial effect on S. aureus，S. albus and B. subtilis. The order of antibacterial effect was rhizome ＞fibrous roots ＞stem and leaves ；the relative contents of β-caryophyllene，α-humulene and modhephene were positively correlated with inhibitory effects of essential oil against S. aureus and B. subtilis（P＜0.05 or P＜0.01）. The relative contents of atractylone ，zingiberene and agarospirol were positively correlated with the inhibitory effect of essential oil against B. subtilis（P＜0.05 or P＜0.01）. CONCLUSIONS There are significant differences in the chemical composition and contents of essential oil from medicinal and non -medicinal parts of A. chinensis，and all of them have antibacterial activity in vitro .
