Correlation and Dynamic Changes between the Polarization of Monocyte-derived Macrophages and HBV DNA in Chronic Hepatitis B
10.13471/j.cnki.j.sun.yat-sen.univ（med.sci）.2022.0111
- VernacularTitle:慢性乙型肝炎患者外周单核细胞衍生的巨噬细胞的极化状态与HBV DNA的相关性及其动态变化
- Author:
Xiao-tong CHEN
1
;
Jing LIANG
1
;
Xiang-fu LIU
2
;
Yu-rong GU
1
;
Yan-yan ZHANG
1
;
Zhe-bin WU
1
;
Xiu-ting HE
3
;
Yu-bao ZHENG
1
Author Information
1. Department of Infectious Diseases， The Third Affiliated Hospital of Sun Yat-sen University， Guangzhou 510630， China
2. Department of Blood Transfusion， The Third Affiliated Hospital of Sun Yat-sen University， Guangzhou 510630， China
3. Guangdong Industry Polytechnic， Guangzhou 510300， China
- Publication Type:Journal Article
- Keywords:
chronic hepatic B;
HBV DNA;
HBeAg;
the palorization of monocyte-derived macrophages
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2022;43(1):86-95
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the dynamic change of monocyte-derived macrophages （MDMs） in chronic hepatitis B （CHB） during the treatment by ETV or TDF， and the association between the polarization of monocyte-derived macrophages （MDMs） and HBV DNA， HBeAg in CHB. MethodsPeripheral blood of 101 patients with chronic hepatitis B and 20 healthy donors were enrolled. MDMs were purified from 86 patients with CHB and 12 healthy donors by peripheral blood mononuclear cell （PBMC）. Flow cytometry was use to analyze the expression of specific markers on polarized macrophages to further evaluate the polarization of MDMs （M1/M2 MDMs）， including CD80， HLA-DR， CD163 and CD206. Additionally， qPCR was used to evaluate the mRNA expression of inflammatory factors of MDMs in 15 patients with chronic hepatitis B and 8 healthy controls. ResultsThe proportion of CD163+CD206+MDMs （M2 MDMs） were reduced in patients with CHB （P = 0.030）， compared with healthy controls. Correspondingly， the mRNA expression of anti-inflammatory factor， IL-10， decreased significantly （P = 0.040）. With the progress of nucleos（t）ide analogs （NAs） therapy， the proportion of CD163+CD206+MDMs （M2 MDMs） increased gradually （P < 0.001）， while the proportion of CD80+HLA-DR+MDMs （M1 MDMs） decreased gradually （P = 0.005）. In addition， M2 MDMs were negatively associated with both HBeAg （P = 0.019）， HBV DNA （P = 0.002）， AST （P = 0.048） and ALT （P = 0.030） in patients with CHB. ROC curve analysis showed that CD163+CD206+MDMs （M2 MDMs） had high predicting value for the virologic response of CHB during NA therapy， and could be used to predict undetectable HBV DNA ［ROC 95％CI： 0.705 （0.594，0.815）］ and HBeAg seroconversion ［ROC 95％CI： 0.740 （0.634，0.847）］. ConclusionsThere are dynamic changes of MDMs polarization in patients with CHB during NA therapy， and the negative correlation between the proportion of M2 MDMs and HBV DNA， HBeAg indicates that M2 MDMs could have a high predicting value in the virologic response of CHB during NA therapy.
