Effects and Mechanisms of Lactobacillus Paracasei on the Prevention of Atherosclerosis
10.13471/j.cnki.j.sun.yat-sen.univ（med.sci）.2022.0107
- VernacularTitle:副干酪乳杆菌抑制动脉粥样硬化进展的作用及其机制
- Author:
Xiao-hui WANG
1
;
Xin-xiu BAI
1
;
Shan-shan ZHU
1
;
Lu-di LIU
1
;
Ya-wen ZHAO
1
;
Jia-lu YANG
1
;
Min XIA
1
Author Information
1. School of Public Health， Sun Yat-sen University， Guangzhou 510080， China
- Publication Type:Journal Article
- Keywords:
Lactobacillus paracasei;
atherosclerosis;
cholesterol uptake;
macrophages
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2022;43(1):51-60
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore the effect and mechanism of Lactobacillus paracasei （Lpa） on the prevention of atherosclerosis. MethodsEight-week-old male ApoE-/- mice were used to establish the atherosclerotic model， and the mice were randomly divided into three groups： ① normal chow control group： ApoE-/- + normal chow diet （n=10）； ② model group： ApoE-/- + western diet + PBS （n=10）； ③ Lactobacillus paracasei intervention group： ApoE-/- + western diet + Lactobacillus paracasei （n=10）. followed by histological evaluations of atherosclerotic lesion in aorta. Immunofluorescence analysis was used to measure the cholesterol accumulation and cholesterol uptake of peritoneal macrophages. Western blot was used to measure the protein expression level of class A scavenger receptor （SR-A） and class B scavenger receptor （CD36）. ResultIntervention of Lactobacillus paracasei can significantly suppress the formation of atherosclerotic plaque in ApoE-/- mice， reduce the inflammatory reaction of arterial blood vessels， inhibit the lipid accumulation and cholesterol uptake of macrophages， and reduce the protein expression of SR-A. ConclusionsLactobacillus paracasei may protect against atherosclerosis by preventing the inflammation of arterial blood vessels and the formation of foam cells by reducing the protein expression of SR-A.
