Histone Deacetylase Inhibitors Suppress Glioma Cell Proliferation by Downregulating MCM2-7 Expression
10.13471/j.cnki.j.sun.yat-sen.univ（med.sci）.2022.0403
- VernacularTitle:组蛋白去乙酰化酶抑制剂通过下调MCM2-7表达抑制神经胶质瘤细胞增殖
- Author:
Hui-feng LI
1
;
Zhong-min YUAN
1
;
Sen-bin WU
1
;
Ying MA
1
;
Fan-yi ZHAO
1
;
Wei-wen HE
1
;
Jian-feng LIANG
1
;
Jian-wei WU
1
Author Information
1. Institute of Neuroscience， The Second Affiliated Hospital of Guangzhou Medical University， Guangzhou 510260， China
- Publication Type:Journal Article
- Keywords:
glioma;
histone deacetylase inhibitors （HDACIs）;
MCM2-7;
cell proliferation
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2022;43(4):530-538
- CountryChina
- Language:Chinese
-
Abstract:
objectiveTo investigate the mechanism of histone deacetylase inhibitors （HDACIs） in suppressing glioma cell proliferation. MethodsGlioma cell lines U251 and H4 were cultured in vitro and treated with HDACIs， including LBH589， M344 and SAHA， MTT assay， flow cytometry， RT-qPCR assay and western blotting were perfomed to determine the cell viability， cell cycle progression， mRNA and protein expression of minichromosome maintenance protein family （MCM2-7）， respectively. BrdU assay was performed to detect the DNA replication in the U251 cells after they were treated with 0.5 µmol/L LBH589 and 0.5 µmol/L TSA. MTT assay and flow cytometry were performed to determine the viability and cell cycle progression of U251 and H4 cells respectively after they were treated with ciprofloxacin （CPX）. ResultsCompared with those in control group， in HDACIs group， the cell viability was significantly decreased； the viability of cells treated with 0.5 µmol/L LBH589 for 12， 16， 24 h was significantly lower and the longer the LBH589 treatment， the lower the viability （P<0.05）. The percentage of U251 and H4 cells in S-phase was significantly lower （P<0.05）. The mRNA and protein expression of MCM2-7 was significantly decreased （P<0.05）. BrdU incorporation rate was lower. The cell viability and S-phase cell percentage of U251 and H4 cells treated with 0.5 mmol/L LBH589 in CPX group were significantly decreased （both P<0.05）. ConclusionHDACIs suppress glioma cell proliferation via downregulating MCM2-7 expression.
