Ethanol extract from Euphorbia humifusa inhibits the malignant biological behaviors of colorectal cancer SW480 cells by regulating the circRHOT1/miR-29a-3p molecular axis
DOI：10.3872/j.issn.1007-385x.2022.09.004
- VernacularTitle:地锦草乙醇提取物通过circRHOT1/miR-29a-3p轴抑制结直肠癌SW480细胞的恶性生物学行为
- Author:
FAN Honggea
1
;
DAI Weibob
1
;
ZHANG Xuefengc
1
Author Information
1. a. Department of Anorectal Medicine; b. Laboratory of Pharmacology of Traditional Chinese Medicine; c. the Third Department of Surgery, Zhongshan Hospital of Traditional Chinese Medicine, Zhongshan 528400, Guangdong, China
- Publication Type:Journal Article
- Keywords:
结直肠癌；SW480细胞；地锦草；乙醇提取物；circRHOT1；miR-29a-3p；增殖；迁移；侵袭
- From:
Chinese Journal of Cancer Biotherapy
2022;29(9):806-812
- CountryChina
- Language:Chinese
-
Abstract:
[摘 要] 目的：探讨地锦草乙醇提取物（EEEH）对人结直肠癌SW480细胞生物学行为的影响及其分子机制。方法：体外培养SW480细胞，实验分为Con组、EEEH-L组、EEEH-M组、EEEH-H组、si-NC组、si-circRHOT1组、EEEH-H+pcDNA组、EEEH-H+pcDNA-circRHOT1组，分别以si-NC、si-circRHOT1、pcDNA、pcDNA-circRHOT1转染SW480细胞，采用CCK-8法、细胞克隆形成实验、Transwell实验分别检测转染后各组细胞的增殖、迁移及侵袭能力，qPCR法检测转染后各组SW480细胞circRHOT1和miR-29a-3p的表达，WB法检测各组细胞中MMP-2、MMP-9蛋白的表达。双荧光素酶报告基因实验检测circRHOT1与miR-29a-3p之间的靶向关系。结果：与Con组比较，EEEH-L组、EEEH-M组、EEEH-H组SW480细胞中MMP-2和MMP-9蛋白表达均明显降低（均P<0.05），circRHOT1的表达均降低（均P<0.05）而miR-29a-3p的表达均升高（均P<0.05）且呈剂量依赖性；细胞的存活率、细胞克隆形成数、迁移及侵袭细胞数均减少（均P<0.05）。circRHOT1可靶向负调控miR-29a-3p的表达。敲减circRHOT1可抑制SW480细胞的增殖、迁移及侵袭能力，而过表达circRHOT1则可减弱EEEH对SW480细胞增殖、迁移及侵袭的抑制作用。结论：EEEH可通过调控circRHOT1/miR-29a-3p轴而抑制结直肠癌SW480细胞的增殖、迁移及侵袭能力。
- Full text:20220904.pdf
