Development of a Luminex xTAG Assay for the Rapid Detection of Common Food Borne Pathogens
- VernacularTitle:xTAG液相芯片技术检测多种食源性致病菌方法的研究
- Author:
Yan PENG
1
;
Bo YU
1
;
Jing-ling ZHANG
1
;
Wen-jing ZOU
1
Author Information
- Publication Type:Journal Article
- Keywords: xTAG Assay; food borne pathogens; MagPlex-TAG Microspheres; Luminex
- From: Journal of Public Health and Preventive Medicine 2022;33(5):54-56
- CountryChina
- Language:Chinese
- Abstract: Objective To develop a bead-based xTAG assay for the rapid detection of Salmonella，Vibrio parahaemolyticus，Listeria monocytogenes and Staphylococcus aureus． Methods Specific primers with xTAG were designed and synthesized based on the target gene sequences and MagPlex-TAG Microspheres . After a multiplex PCR amplification，the PCR products hybridized with MagPlex-TAG Microspheres． The Luminex200 array system was used to detect the results of hybridization． The sensitivity and specificity of the method was valued． Results The Luminex xTAG Assay was developed for a specific，rapid，accurate detection of Salmonella，Vibrio parahaemolyticus，Listeria monocytogenes and Staphylococcus aureus. The sensitivity of detection of toxR gene of Salmonella，hly gene of Vibrio parahaemolyticus，and invA gene of Listeria monocytogenes was 100 CFU ／mL． The sensitivity of detection of Sa442 gene of Staphylococcus aureus was 1×103CFU／mL． Conclusion The Luminex xTAG Assay could rapidly, accurately and specifically detect four foodborne pathogens Salmonella，Vibrio parahaemolyticus，Listeria monocytogenesand, Staphylococcus aureus.
