Establishment of an animal model of Sparganum mansoni infection and study on therapeutic methods II Establishment of a mouse model of sparganosis mansoni via oral administration of procercoids
10.16250/j.32.1374.2021142
- VernacularTitle:曼氏裂头蚴病动物模型的建立及诊治技术研究 Ⅱ原尾蚴灌胃法建立曼氏裂头蚴病小鼠模型
- Author:
Xian-shi TANG
1
;
Yong-liang XU
1
;
Qiang WANG
1
;
Ying ZHANG
1
;
De-sheng TONG
1
Author Information
1. Key Laboratory of National Health Commission on Parasitic Disease Control and Prevention; Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology; Jiangsu Institute of Parasitic Diseases, Wuxi, Jiangsu 214064, China
- Publication Type:Journal Article
- Keywords:
Sparganosis mansoni;
Sparganum mansoni;
Procercoid;
Animal model
- From:
Chinese Journal of Schistosomiasis Control
2022;34(4):400-403
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an animal model of sparganosis mansoni through oral administration of Cyclops infected with procercoids. Methods Domestic cats were infected with Sparganum mansoni under laboratory conditions, and fresh cat stool samples were collected, washed in dechlorinated water, and filtered. Spirometra mansoni eggs were collected and prepared into suspensions. Twenty C57BL/6j mice were randomly divided into the experimental group (n = 15) and the control group (n = 5). Wild Cyclops were infected with Spirometra mansoni coracidia to allow 3 to 5 procercoids in each Cyclop. Then, each mouse in the experimental group was given 15 Cyclops infected with procercoids by gavage, while mice in the control group were orally administered with the same volume of dechlorinated water. All mice were sacrificed after 5 months, and dissected, and suspicious Sparganum mansoni worms were collected. The serum specific IgG antibody against Sparganum mansoni was measured in mice using enzyme-linked immunosorbent assay (ELISA). Genomic DNA was isolated from suspicious Sparganum mansoni worms, and the specific Sparganum mansoni cytochrome oxidase I (COI) gene was amplified using PCR assay. Results Among the 15 mice in the experimental group, six were positive for the serum specific IgG antibody against Sparganum mansoni, and milky white worms were found and collected from the subcutaneous regions of 4 out of 6 mice. Only one worm was detected in each mouse, and the worm morphology was similar to Sparganum mansoni. Capillary electrophoresis of the PCR amplification products of COI gene presented a specific band with 151 bp in size, and sequencing analysis revealed 100% homology with Sparganum mansoni. Conclusions A mouse model of sparganosis mansoni is successfully created through oral administration of Cyclops infected with Spirometra mansoni procercoids.
